Background: The Oryza sativa L. indica subspecies is the most widely cultivated rice. During the last few years, we have collected over 20,000 putative full-length cDNAs and over 40,000 ESTs isolated from various cDNA libraries of two indica varieties Guangluai 4 and Minghui 63. A database of the rice indica cDNAs was therefore built to provide a comprehensive web data source for searching and retrieving the indica cDNA clones.
Results: Rice Indica cDNA Database (RICD) is an online MySQL-PHP driven database with a user-friendly web interface. It allows investigators to query the cDNA clones by keyword, genome position, nucleotide or protein sequence, and putative function. It also provides a series of information, including sequences, protein domain annotations, similarity search results, SNPs and InDels information, and hyperlinks to gene annotation in both The Rice Annotation Project Database (RAP-DB) and The TIGR Rice Genome Annotation Resource, expression atlas in RiceGE and variation report in Gramene of each cDNA.
Conclusion: The online rice indica cDNA database provides cDNA resource with comprehensive information to researchers for functional analysis of indica subspecies and for comparative genomics. The RICD database is available through our website http://www.ncgr.ac.cn/ricd.
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http://dx.doi.org/10.1186/1471-2229-8-118 | DOI Listing |
Plants (Basel)
December 2024
State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Yield-related traits have higher heritability and lower genotype-by-environment interaction, making them more suitable for genetic studies in comparison with the yield per se. Different populations have been developed and employed in QTL mapping; however, the use of reciprocal SSSLs is limited. In this study, three kinds of bi-parental populations were used to investigate the stable and novel QTLs on six yield-related traits, i.
View Article and Find Full Text PDFFront Plant Sci
December 2024
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, China.
Since the inception of hybrid rice technology 50 years ago, it has not only substantially increased rice yield per unit area but also expedited the development of high-quality rice varieties. However, the evolutionary characteristics of hybrid rice quality remain unclear. To address this gap, it is imperative to leverage more representative and comprehensive hybrid rice resources to analyze phenotypic variation diversity and its primary genetic basis, thereby offering more efficient guidance for molecular breeding.
View Article and Find Full Text PDFEnviron Sci Technol
January 2025
Institute of Soil and Water Resources and Environmental Science, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China.
Cadmium (Cd) is efficiently transferred from soil to food crops, notably rice. Research indicates that indica rice grains may accumulate more Cd than japonica cultivars; however, differences in Cd bioavailability (the fraction of ingested rice Cd absorbed into the systemic circulation) and toxicity remain unexplored, thus hindering a comprehensive understanding of exposure and health risks. To address this, a mouse bioassay was conducted to evaluate the relative bioavailability (RBA) of Cd in 35 samples each of japonica and indica rice, determining which type exhibits lower Cd bioavailability.
View Article and Find Full Text PDFPlants (Basel)
December 2024
Key Laboratory of Plant Functional Genomics of the Ministry of Education/Zhongshan Biological Breeding Laboratory, Yangzhou University, Yangzhou 225009, China.
The whiteness of rice grains (WRG) is a key indicator of appearance quality, directly impacting its commercial value. The trait is quantitative, influenced by multiple factors, and no specific genes have been cloned to date. In this study, we first examined the correlation between the whiteness of polished rice, cooked rice, and rice flour, finding that the whiteness of rice flour significantly correlated with both polished and cooked rice.
View Article and Find Full Text PDFInt J Mol Sci
December 2024
Rice Research Institute, Guangdong Academy of Agricultural Sciences/South China High-Quality Rice Breeding Laboratory (Jointly Established by Ministry of Agriculture and Rural Affairs and Provincial Government)/Guangdong Key Laboratory of Rice Science and Technology/Guangdong Rice Engineering Laboratory, Guangzhou 510640, China.
Rice is a short-day thermophilic crop that originated from the low latitudes of the tropics and subtropics; it requires high temperatures for growth but is sensitive to low temperatures. Therefore, it is highly important to explore and analyze the molecular mechanism of cold tolerance in rice to expand rice planting areas. Here, we report a phenotypic evaluation based on low-temperature stress in indica rice (R998) and wild rice (GZW) and a comparative transcriptomic study conducted at six time points.
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