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Antimicrobial Resistance Profiles of Isolated from Food Animal Carcasses During 2010-2023 in South Korea.
Foodborne Pathog Dis
January 2025
Department of Animal and Plant Quarantine Agency, Bacterial Disease Division, Gimcheon-si, Republic of Korea.
Antimicrobial-resistant bacterial contamination of meat poses a significant global public health risk. We aimed to determine antimicrobial resistance profiles and trends of recovered from carcasses of healthy food-producing animals in South Korea during 2010-2023. In total, 4748 isolates obtained from cattle ( = 1582), pigs ( = 1572), and chickens ( = 1594) were assessed for susceptibility to 12 antimicrobials.
View Article and Find Full Text PDFA Cost-Effective Nonaqueous Reversed-Phase High-Performance Liquid Chromatography Method to Measure Vitamin D3 in Hen's Egg Yolk.
J Sep Sci
January 2025
Department of Pharmaceutical Sciences, University of Perugia, Perugia, Italy.
The objective of this study is to develop an HPLC-UV method for the cost-effective and quantitative determination of vitamin D3 in food, even in the presence of vitamin D2, with a specific focus on egg yolk. During method development, the performance of three stationary phases in resolving the peak of vitamin D2 from that of vitamin D3 was investigated. The physicochemical properties of these phases differed particularly in the extent of hydrophobicity and silanophilic activity, including a GraceSmart RP C18 column without silanol endcapping, a Robusta RP C18 column with silanol endcapping, and a Waters Xbridge RP C18 column with ethylene-bridged hybrid (BEH) particle technology.
View Article and Find Full Text PDFHA198 mutations in H9N2 avian influenza: molecular dynamics insights into receptor binding.
Front Vet Sci
January 2025
Jiangsu Agri-animal Husbandry Vocational College, Taizhou, Jiangsu, China.
Introduction: The H9N2 avian influenza virus is widely disseminated in poultry and poses a zoonotic threat, despite vaccination efforts. Mutations at residue 198 of hemagglutinin (HA) are critical for antigenic variation and receptor-binding specificity, but the underlying molecular mechanisms remain unclear. This study explores the molecular mechanisms by which mutations at the HA 198 site affect the antigenicity, receptor specificity, and binding affinity of the H9N2 virus.
View Article and Find Full Text PDFSe-methylselenocysteine inhibits inflammatory response in an LPS-stimulated chicken HD11 macrophage-like cell model through the NFKB2 pathway.
Front Vet Sci
January 2025
College of Animal Science and Technology, Yangtze University, Jingzhou, China.
Among the various sources of selenium supplementations, the Se-methylselenocysteine (SeMC) is a natural organic selenium compound that has been demonstrated to have multiple advantages in terms of metabolism efficiency and biosafety in animals. Nevertheless, the genome-wide impact of SeMC on gene transcription remains to be elucidated. In this study, we employed an LPS-stimulated chicken HD11 macrophage-like cell model to identify the principal transcription factors involved in transcriptome regulation responsible for SeMC treatment.
View Article and Find Full Text PDFHyperthermia suppresses the biological characteristics and migration of chicken primordial germ cells.
Front Genome Ed
January 2025
State Key Laboratory of Animal Biotech Breeding, China Agricultural University, Beijing, China.
Primordial germ cells (PGCs) play a crucial role in transmitting genetic information to the next-generation. In chickens, genetically edited PGCs can be propagated and subsequently transplanted into recipient embryos to produce offspring with desired genetic traits. However, during early embryogenesis, the effects of external conditions on PGC migration through the vascular system to the gonads have yet to be explored, which may affect the efficiency of preparing gene-edited chickens.
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