Purpose: To study thermal variations obtained through infrared image in rats, and to evaluate the relationship between intestinal ischemic time and histopathological findings.
Methods: Thirty Wistar rats were operated after distribution in 5 groups with different times of ischemia. Thermograms were obtained by using a infrared camera. The surgical technique has been standardized for all groups: abdominal cavity opening by a 5 cm length incision in the midline, abdominal wall plans section and cavity exposure, and exteriorization of the intestine. In group I (control), it was proceeded only laparotomy without superior mesenteric artery ligature. After first thermogram done, incision was closed with continuing suture. In each rat in groups II, III, IV and V, the superior mesenteric artery was located at its origin on abdominal aorta, dissected and occluded with a vascular microclamp, subjecting the intestine to ischemia in variable times.
Results: Rats submitted to a 30 minutes ischemia presented reactive hyperemia, thermal differential of 1.8 degrees C and normal pathological examination. The 1 hour ischemia produced reactive hyperemia with ischemic areas, thermal differential of 1.0 degrees C and injuries at villosities' tips. However, the 90 minutes ischemia had not shown reactive hyperemia with large ischemic areas, thermal differential of -1.0 degrees C and injury in the upper third of the villosities. The 2 hours ischemia demonstrated a severe ischemia, thermal differential of -2.0 degrees C and injury throughout the all villosities' extension.
Conclusion: It has been possible studying thermal variations through infrared image in rats, showing correlation between thermal response in thermograms, ischemic time and histopathological findings.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1590/s0102-86502008000600008 | DOI Listing |
J Fungi (Basel)
November 2024
Key Laboratory of Microbiological Metrology, Measurement & Bio-Product Quality Security, State Administration for Market Regulation, College of Life Sciences, China Jiliang University, Hangzhou 310018, China.
is a dimorphic fungus that specifically infects , causing stem swelling and the formation of an edible fleshy stem known as jiaobai. The pathogenicity of is closely associated with the development of jiaobai and phenotypic differentiation. Msb2 acts as a key upstream sensor in the MAPK (mitogen-activated protein kinase) signaling pathway, playing critical roles in fungal hyphal growth, osmotic regulation, maintenance of cell wall integrity, temperature adaptation, and pathogenicity.
View Article and Find Full Text PDFBiosensors (Basel)
November 2024
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
Point-of-care (POC) immunoassays have become convincing alternatives to traditional immunosensing methods for the sensitive and real-time detection of targets. Immunoassays based on gas-generating reactions were recently developed and have been used in various fields due to their advantages, such as rapid measurement, direct reading, simple operation, and low cost. Enzymes or nanoparticles modified with antibodies can effectively catalyze gas-generating reactions and convert immunorecognition events into gas pressure signals, which can be easily recorded by multifunctional portable devices.
View Article and Find Full Text PDFBiomimetics (Basel)
December 2024
Department of Mechanical Engineering and Aeronautics, University of Patras, 26504 Patras, Greece.
3D-printed biomedical polylactic acid (PLA) scaffolds were developed, and their biodegradation, as well as their thermomechanical behavior, were studied in a relevant in vitro environment. The scaffold's biodegradability profile has been monitored after immersion in a cell culture medium that contains components of blood and body fluids. Two types of biodegradation experiments were performed-a standard static one and an adapted stirring one, mimicking the body fluids' flow, respectively-to achieve a comparative investigation.
View Article and Find Full Text PDFAnal Bioanal Chem
December 2024
Division of Pulmonary, Critical Care and Sleep Medicine, University of California, Davis, Sacramento, CA, USA.
Metabolically active cells emit volatile organic compounds (VOCs) that can be used in real time to non-invasively monitor the health of cell cultures. We utilized these naturally occurring VOCs in an adapted culture method to detect differences in culturing Chinese hamster ovary (CHO) cells with and without Staphylococcus epidermidis and Aspergillus fumigatus contaminations. The VOC emissions from the cell cultures were extracted and measured from the culture flask headspace using polydimethylsiloxane (PDMS)-coated Twisters, which were subjected to thermal desorption-gas chromatography-mass spectrometry (TD-GC-MS) analysis.
View Article and Find Full Text PDFFood Sci Nutr
December 2024
Laboratory 13 Thermal and Structural Analysis of Materials and Foods National Autonomous University of Mexico-Superior Studies Multidisciplinary Research Unit, Faculty at Cuautitlan (UNAM-FESC), Campus 4 Cuautitlan Izcalli Mexico.
Meat preservation processes have been widely studied over time, especially those related to low temperatures (freezing and freeze-drying); however, there is very little research that directly relates the effect of these processes on the structure of meat-and the main meat proteins-and how these changes affect some attributes of the final quality. Pork loin meat () was used, which was frozen-thawed and freeze-dried-rehydrated to subsequently evaluate changes in its chemical composition and physicochemical parameters such as water activity ( ), pH, and water-holding capacity. Physical aspects such as color profile, surface myoglobin fraction, shear force, and histological sections were also evaluated, along with thermal analysis by modulated differential scanning calorimetry.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!