The objective of this study was to test an approach that combines bioinformatic and subcellular localization analysis to identify novel cell wall protein genes in Arabidopsis. Proteins with unknown function in the Arabidopsis genome were first identified and scanned for the presence of N-terminal signal peptides. The signal peptide-containing function-unknown proteins were further analyzed to eliminate the ones containing other sequences, such as endoplasmic reticulum and vacuole retention signals, that may prevent a protein from secretion into cell walls. The top ten genes passing the bioinformatic analysis were selected for protein subcellular localization using green fluorescence protein (GFP) as a reporter. A vector was constructed for high throughput gene-GFP fusion protein generation and overexpression in Arabidopsis for gene function analysis. Transformants of six genes showed reasonable expression of GFP fusion protein. However, none of the transformants showed GFP localization in cell walls. The low rate of new cell wall protein discovery suggests that the number of unidentified cell wall proteins in the Arabidopsis genome may be small.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.plaphy.2008.10.003 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
[PLSR model based on near-infrared spectroscopy for the detection of wood fiber anatomy of ].
Ying Yong Sheng Tai Xue Bao
October 2024
Research Institute of Subtropical Forestry, Chinese Academy of Forestry/Zhejiang Key Laboratory of Forest Genetics and Bree-ding, Hangzhou 311400, China.
To rapidly acquire fiber phenotypic data for wood quality assessment, we used a portable NIR spectro-meter to collect spectral data in 100 individuals of at 18-year-old of 20 different provenances, and simultaneously collected wood cores. Wood basic density and the anatomical structure of wood fiber were measured. The standard normal variate (SNV), orthogonal signal correction (OSC), and multiplicative scatter correction (MSC) methods were used for spectral preprocessing, the competitive adaptive reweighted sampling (CARS) method were used for wavelength selection, and the partial least squares regression (PLSR) model were established.
View Article and Find Full Text PDFFurther confirmation of a highly prognostic grading scheme based upon tumour budding and cell cluster size in cervical squamous cell carcinoma.
Histopathology
December 2024
Department of Surgical Pathology and Center for Uterine Cancer Diagnosis and Therapy Research of Zhejiang Province, Womens Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China.
Aims: Our study aimed to further confirm the clinical significance of the tumour budding activity and cell nest size-based (TBNS) grading scheme in cervical squamous cell carcinomas (SCC).
Methods And Results: We applied the TBNS system to assess the prognostic value in an institutional cohort of well-annotated cervical SCC consisting of 312 consecutive cases with surgical resection, no neoadjuvant chemotherapy and higher than stage pT1a. We found that high budding activity, single cell and TBNS grade 3 were more frequently associated with a decreased overall survival (OS) time and disease-free survival (DFS) time (P < 0.
The Malectin-like kinase gene MdMDS1 negatively regulates the resistance of Pyrus betulifolia to Valsa canker by promoting the expression of PbePME1.
Physiol Plant
December 2024
College of Horticulture, Gansu Agricultural University, Lanzhou, China.
Valsa canker, caused by fungal pathogens in Valsa species, is a fungal disease of apple and pear growing in China and even in Asia. Malectin-like kinases play crucial roles in plant recognition of the pathogen-induced signals and subsequent activation of partially host immune responses. However, the role of MEDOS1 (MDS1), a Malectin-like kinase, in plant immunity has not yet been extensively explored.
View Article and Find Full Text PDFMultidimensional Characterization of the Physiological State of Hematococcuspluvialis Using Scanning Structured Illumination Super-Resolution Microscopy.
Anal Chem
December 2024
Key Laboratory of Optoelectronic Devices and Systems of the Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China.
(HP) is a freshwater alga known for its ability to accumulate the potent antioxidant astaxanthin, which has extensive applications in aquaculture, pharmaceuticals, and cosmetics. Astaxanthin rapidly accumulates under unfavorable environmental conditions. However, the mechanisms of astaxanthin accumulation under various stress conditions remain unclear.
View Article and Find Full Text PDFProtective Role of TAOK1-CDC20 Axis in Atherosclerosis: Modulation of Endothelial Apoptosis and Senescence.
Discov Med
December 2024
Department of Cardiology, Jiangxi Provincial People's Hospital, The First Affiliated Hospital of Nanchang Medical College, 330006 Nanchang, Jiangxi, China.
Background: Atherosclerosis, a chronic inflammatory condition characterized by the accumulation of lipid and fibrous elements in the arterial wall, is a major contributor to cardiovascular disease. This study aimed to investigate the regulation of apoptosis and cellular aging in human umbilical vein endothelial cells by Thousand and One Amino Acid Kinase 1 (TAOK1) via Cell division cycle 20 () in the context of atherosclerosis.
Methods: The study evaluated the impact of TAOK1 on Oxidized low-density lipoprotein (ox-LDL)-induced changes in cell viability, angiogenesis, cell senescence, apoptosis, cell cycle arrest, and related signaling pathways in human umbilical vein endothelial cells (HUVECs) using Cell Counting Kit-8, β-galactosidase staining, flow cytometry, and western blot.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!