AI Article Synopsis
- The study aimed to compare the bacterial profiles in the root canals of patients with chronic apical periodontitis from Brazil and Norway using DGGE and RISA techniques.
- DNA extracted from root canal samples was analyzed to create banding patterns showing the bacterial communities present.
- Results indicated significant individual variability in bacterial profiles, but some similarities suggested a geographic influence on the microbiota.
Article Abstract
The aim of this study was to compare the bacterial community profiles of the root canal microbiota associated with chronic apical periodontitis from Brazilian and Norwegian patients using the denaturing gradient gel electrophoresis (DGGE) and the ribosomal intergenic spacer analysis (RISA) approaches. DNA extracted from root canal samples was subjected to polymerase chain reaction using primers appropriate for further DGGE or RISA analysis. The resulting banding patterns representative of the bacterial community structures in samples from the two locations were compared. DGGE and RISA fingerprints showed a great interindividual variability in the bacterial community profiles, irrespective of the geographic location of the patient. However, similarities among the bacterial community DGGE profiles revealed the existence of a geography-related pattern.
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| http://dx.doi.org/10.1016/j.joen.2008.08.037 | DOI Listing |
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