[Expression pattern of polycomb gene Nspc1 at the early developmental stage in zebrafish].

Zhongguo Yi Xue Ke Xue Yuan Xue Bao

National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.

Published: October 2008

AI Article Synopsis

  • The study aimed to investigate the expression pattern of the Polycomb gene Nspc1 during the early developmental stages of zebrafish.
  • Different stages of zebrafish embryos were analyzed using in situ hybridization to visualize Nspc1 expression.
  • Results showed that Nspc1 is globally expressed early on, with more specific expression in the nervous system beginning at the somites stage, suggesting its importance in zebrafish development.

Article Abstract

Objective: To study the expression pattern of Polycomb gene Nspc1 at the early developmental stage in zebrafish.

Methods: In situ hybridization probe for Nspc1 was designed according to the GenBank information. Collecting zebrafish embryos at different stages including one cell stage, two-cell stage, bud stage, and somites stage, we hybridized them with the prepared probe. Then the hybridization signals at different intervals were observed and photographed at the right time.

Results: Nspc1 was expressed globally at the early stage. Its expression specificity began at the somites stage, mainly in the nervous system of the head.

Conclusion: Nspc1 may play essential roles in the early stage development of zebrafish, especially in the nervous system.

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