We compared two technologies of real-time PCR (with the use of fluorescent SYBR Green I dye and specific TaqMan probe) for quantification of the dose of her2 gene in breast tumors. The maximum increase in the gene dose in TaqMan and SYBR Green I analyses was 10- and 5-fold, respectively. In was found that TaqMan and SYBR Green I technologies allow detection of the matrix in amounts corresponding to 1-100 and 2.5-40.0 ng genomic DNA, respectively. Tenfold increase in the gene dose leads to incorrect evaluation of multiplication ratio in the SYBR Green I analysis. These results suggest that TaqMan technology is more preferable for correct evaluation of her2 gene dose.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s10517-008-0060-3 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Development of qPCR methods to detect and quantify the novel Fusarium graminearum 3ANX chemotype variant.
J Microbiol Methods
January 2025
Charlottetown Research and Development Centre, Agriculture and Agri-Food Canada, 440 University Avenue, Charlottetown, PE C1A 4N6, Canada. Electronic address:
The devastating plant pathogen Fusarium graminearum produces mycotoxins including the novel 3ANX toxin. To detect 3ANX-producing isolates, SYBR Green and locked nucleic acid probe assays were developed, targeting 3ANX Tri1 polymorphisms. Assays were efficient with R > 0.
View Article and Find Full Text PDFCopy number variations at the locus and their relationship with resistance to soybean cyst nematode ().
Front Plant Sci
December 2024
Department of Plant Pathology, North Dakota State University, Fargo, ND, United States.
Soybean cyst nematode (SCN, ) is a devastating pest affecting soybean production worldwide. Host resistance is one of the primary practices used to manage SCN. The locus contributes to the strong and effective SCN resistance, with resistance levels predominantly governed by copy number variations (CNVs) and, to lesser extent, sequence variations.
View Article and Find Full Text PDFDNA methylation of long noncoding RNA cytochrome B in diabetic retinopathy.
Noncoding RNA Res
April 2025
Kresge Eye Institute, Wayne State University, Detroit, MI, USA.
Diabetic retinopathy, a microvascular complication of diabetes, is the leading cause of blindness in adults, but the molecular mechanism of its development remains unclear. Retinal mitochondrial DNA is damaged and hypermethylated, and mtDNA-encoded genes are downregulated. Expression of a long noncoding RNA (larger than 200 nucleotides, which does not translate into proteins), encoded by mtDNA, cytochrome B (Lnc), is also downregulated.
View Article and Find Full Text PDFDevelopment of a qPCR assay for early detection and quantification of in kiwifruit plant and soil affected by Vine Decline Syndrome.
Plant Dis
January 2025
University of Torino, DISAFA - Dept. Agricultural, Forestry and Food Sciences, Largo Braccini 2, Grugliasco, TO, Italy, 10095.
Kiwifruit Vine Decline Syndrome (KVDS) is a soilborne disease affecting Actinidia fruit trees in perennial cropping systems. Since its emergence in 2012, studies have increasingly identified the oomycete as a major causative agent of the disease. is also implicated in complex soilborne disease systems of woody perennial crops, including replant disease in apple and pear.
View Article and Find Full Text PDFAberrant Methylation of is Associated with the Pathogenesis of Autoimmune Pancreatitis.
Int J Surg Pathol
January 2025
Department of Laboratory Medicine, Shinshu University School of Medicine, Matsumoto, Japan.
IgG4-related disease (IgG4-RD) is an autoimmune disease of unknown cause. is a transcription factor involved in immune responses, and its dysfunction leads to uncontrolled immune responses. We performed, to our knowledge, the first methylation analysis in type 1 autoimmune pancreatitis (denoted simply as AIP), a representative IgG4-RD.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!