AI Article Synopsis
- Dcytb is an important ferric reductase highly present in duodenal enterocytes, playing a crucial role in iron absorption due to its iron regulation.
- Expression of Dcytb in cell models increases ferric reductase activity and enhances iron uptake, with additional cupric reductase activity observed in MDCK cells.
- Research using Dcytb-knockout mice shows that it is the sole iron-regulated ferric reductase in the duodenal mucosa, and its absence negatively impacts iron absorption.
Article Abstract
Dcytb (duodenal cytochrome b) is an iron-regulated ferric reductase highly expressed in duodenal enterocytes. Its location and strong regulation by iron has indicated it plays an important role in iron absorption. Expression of Dcytb in cells (Caco-2 and MDCK) was found to increase both ferric reductase activity and stimulate uptake of (59)Fe. An additional increase in cupric reductase activity was found in MDCK (Madin-Darby canine kidney) cells expressing Dcytb. Expression and purification of Dcytb in insect cells reveals that Dcytb is a di-haem protein and that the haems are reducible by ascorbate, indicating that ascorbate is the likely intracelluar electron donor. Studies underway in Dcytb-knockout mice reveal that Dcytb is the only iron-regulated ferric reductase in the duodenal mucosa and that loss of Dcytb affects iron absorption.
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| http://dx.doi.org/10.1042/BST0361239 | DOI Listing |
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