In vitro studies have proved the presence of epitopes of CD59 in the surface of trophozoites of Entamoeba histolytica (E. histolytica). However, it has not been proved if CD59 molecules are expressed in the surface during the trophozoites' tissue invasion. The aim of the present study was to determine whether the complement-regulatory protein CD59 is present on trophozoites of E. histolytica in human colon. Eleven specimens of amoebic colitis were studied by immunohistochemistry and electron microscopy techniques with a monoclonal antibody against human CD59 molecule. Our results show that a CD59-like molecule is expressed in trophozoites of E. histolytica found in colonic amebic lesions. Also, a CD59-like molecule was detected by western blot analysis in whole lysate of E. histolytica as well as on the plasma membrane by immunocytochemistry. These results suggest that E. histolytica can use CD59-like protein against the lytic action of membrane attack complex.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s00436-008-1262-3 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Eudiplozoon nipponicum (Monogenea, Diplozoidae) and its adaptation to haematophagy as revealed by transcriptome and secretome profiling.
BMC Genomics
April 2021
Department of Botany and Zoology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37, Brno, Czech Republic.
Background: Ectoparasites from the family Diplozoidae (Platyhelminthes, Monogenea) belong to obligate haematophagous helminths of cyprinid fish. Current knowledge of these worms is for the most part limited to their morphological, phylogenetic, and population features. Information concerning the biochemical and molecular nature of physiological processes involved in host-parasite interaction, such as evasion of the immune system and its regulation, digestion of macromolecules, suppression of blood coagulation and inflammation, and effect on host tissue and physiology, is lacking.
View Article and Find Full Text PDFHide and Seek: How Lyme Disease Spirochetes Overcome Complement Attack.
Front Immunol
September 2016
Institute of Medical Microbiology and Infection Control, University Hospital of Frankfurt, Frankfurt am Main , Germany.
Overcoming the first line of the innate immune system is a general hallmark of pathogenic microbes to avoid recognition and to enter the human host. In particular, spirochetes belonging to the sensu lato complex have developed various means to counter the immune response and to successfully survive in diverse host environments for a prolonged period of time. In regard to complement resistance, utilize a plethora of immune evasion strategies involves capturing of host-derived complement regulators, terminating complement activation as well as shedding of cell-destroying complement complexes to manipulate and to expeditiously inhibit human complement.
View Article and Find Full Text PDFFirst insight into CD59-like molecules of adult Fasciola hepatica.
Exp Parasitol
September 2014
Department of Agricultural Sciences and Centre for AgriBioscience, La Trobe University, Bundoora, Victoria 3086, Australia. Electronic address:
The present study focussed on investigating CD59-like molecules of Fasciola hepatica. A cDNA encoding a CD59-like protein (termed FhCD59-1) identified previously in the membrane fraction of the F. hepatica tegument was isolated.
View Article and Find Full Text PDFOn the three-finger protein domain fold and CD59-like proteins in Schistosoma mansoni.
PLoS Negl Trop Dis
June 2014
Centro de Biotecnologia, Instituto Butantan, São Paulo, Brazil.
Background: It is believed that schistosomes evade complement-mediated killing by expressing regulatory proteins on their surface. Recently, six homologues of human CD59, an important inhibitor of the complement system membrane attack complex, were identified in the schistosome genome. Therefore, it is important to investigate whether these molecules could act as CD59-like complement inhibitors in schistosomes as part of an immune evasion strategy.
View Article and Find Full Text PDFTrophozoites of Entamoeba histolytica express a CD59-like molecule in human colon.
Parasitol Res
March 2009
Departamento de Morfología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Av Universidad 940, col. Cd. Universitaria, Aguascalientes CP. 20100, México.
In vitro studies have proved the presence of epitopes of CD59 in the surface of trophozoites of Entamoeba histolytica (E. histolytica). However, it has not been proved if CD59 molecules are expressed in the surface during the trophozoites' tissue invasion.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!