AI Article Synopsis
- The study focused on analyzing the rDNA ITS sequences in Bupleurum medicinal plants to identify DNA markers related to their medicinal properties.
- It was found that the alignment of ITS sequences among Bupleurum plants and their related species was below 75%, indicating significant variation.
- The research concluded that ITS sequences are effective as molecular markers for identifying Radix Bupleuri.
Article Abstract
To determine the variation of rDNA ITS sequences in medicinal plants of the genus Bupleurum and identify corresponding DNA molecular markers associated with medicinally important members, ITS regions were amplified, sequenced, and analyzed by DNAssist version 2.2. We found that the homologous alignment of ITS sequences between members of the genus Bupleurum and associated out-groups was lower than 75%, while within-group alignment was greater than 87%. The conclusion can be drawn that ITS sequences can be used as reliable molecular markers for the identification of Radix Bupleuri.
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| http://dx.doi.org/10.1055/s-0028-1088334 | DOI Listing |
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