AI Article Synopsis
Article Abstract
Conclusion: Glucocorticoid (GC) administration enhanced apoptotic changes in mature olfactory receptor neurons (ORNs). GC administration may enhance regeneration of olfactory epithelium (OE).
Objectives: The mechanism underlying olfactory epithelial cells turnover involves apoptosis replaced by new ORNs. On regeneration of OE, we evaluated the apoptotic changes in OE. Our aim was to corroborate the enhancement of apoptosis of ORNs induced by GCs that are generally administered locally or systemically to patients with olfactory dysfunction.
Materials And Methods: For the in vitro study, we established cultured murine ORNs. Triamcinolone acetonide was added to culture supernatants. ORNs were then cultured for another 2 weeks. In the in vivo study, triamcinolone acetonide was administered to mice 5 or 10 times. The mice were dissected 3 days after the final injection, and the olfactory regions were removed and embedded in paraffin. All samples were examined by immunohistochemical staining and the TdT-mediated dUTP-biotin nick-end labeling (TUNEL) method.
Results: Glucocorticoid receptor (GR) expression of cultured murine ORNs was observed among ORNs at the mature stage. Expression of GRs by murine OE was localized on mature ORNs and supporting cells. Administration of GC to both cultured ORNs and mice resulted in proportions of apoptotic cells that were significantly higher than those in the control groups.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1080/00016480802530663 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Protocol for single sensillum recording from labial olfactory sensory fields in spotted lanternfly.
STAR Protoc
December 2024
Department of Entomology, The Connecticut Agricultural Experiment Station, New Haven, CT 06511, USA. Electronic address:
Article Synopsis
- * A detailed protocol is provided for conducting single sensillum recordings from SLF labial ORNs to assess their sensitivity, specificity, and response patterns to different odors.
- * The protocol includes steps for preparing odorant cartridges, setting up equipment, and analyzing the resulting neuronal data, with further information referenced from Dweck and Claire.
Prosaposin/Saposin Expression in the Developing Rat Olfactory and Vomeronasal Epithelia.
J Dev Biol
November 2024
Laboratory of Veterinary Anatomy, Joint Graduate School of Veterinary Science, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Prosaposin is a glycoprotein widely conserved in vertebrates, and it acts as a precursor for saposins that accelerate hydrolysis in lysosomes or acts as a neurotrophic factor without being processed into saposins. Neurogenesis in the olfactory neuroepithelia, including the olfactory epithelium (OE) and the vomeronasal epithelium (VNE), is known to occur throughout an animal's life, and mature olfactory neurons (ORNs) and vomeronasal receptor neurons (VRNs) have recently been revealed to express prosaposin in the adult olfactory organ. In this study, the expression of prosaposin in the rat olfactory organ during postnatal development was examined.
View Article and Find Full Text PDFComplement Component 3 Promotes Regeneration of Olfactory Receptor Neurons.
Lab Invest
November 2024
Department of Otorhinolaryngology-Head and Neck Surgery, Wakayama Medical University, Wakayama, Japan. Electronic address:
Olfactory receptor neurons (ORNs) in the olfactory epithelium are characterized by high regenerative capacity even after birth, but the molecular mechanisms involved in ORN regeneration remain unclear. Complement component 3 (C3) has been shown to promote tissue regeneration, so we hypothesized that C3 activates innate immunity and also promotes the regeneration of ORNs. In this study, we investigate the role of C3 in ORN regeneration.
View Article and Find Full Text PDFTargeted deletion of olfactory receptors in via CRISPR/Cas9-mediated LexA knock-in.
J Neurogenet
September 2024
Department of Neurobiology, University of California San Diego, La Jolla, CA, USA.
The study of olfaction in has greatly benefited from genetic reagents such as olfactory receptor mutant lines and GAL4 reporter lines. The CRISPR/Cas9 gene-editing system has been increasingly used to create null receptor mutants or replace coding regions with GAL4 reporters. To further expand this toolkit for manipulating fly olfactory receptor neurons (ORNs), we generated null alleles for 11 different olfactory receptors by using CRISPR/Cas9 to knock in LexA drivers, including multiple lines for receptors which have thus far lacked knock-in mutants.
View Article and Find Full Text PDFStimulus duration encoding occurs early in the moth olfactory pathway.
Commun Biol
October 2024
Department of Sensory Ecology, Institute of Ecology and Environmental Sciences of Paris, INRAE, Sorbonne Université, CNRS, IRD, UPEC, Université de Paris, Route de Saint Cyr, Versailles, 78000, France.
Article Synopsis
- Scientists studied how insects like moths and fruit flies use smells (pheromones) to find their way and communicate.
- They created a special device that sends short bursts of pheromones to see how the insect's sensory cells react.
- Moths can recognize when the smell stops, but fruit flies can't do this well because they only need to communicate over short distances.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!