The prevalence of intra-mammary infections in dairy herds was studied in Hamdallaye, Niger. A total of 956 milk samples were collected in 2007 from 239 lactating cows of four local breeds in eight traditional herds; the first sampling was undertaken in the dry season at morning milking, and the second in the rainy season at evening milking. Staphylococcus aureus, Coagulase-Negative Staphylococci (CNS) and environmental microorganisms were detected in significantly (p < 0.05) more samples in the rainy season, 55.2%, than in the dry season, 27.1%. Statistically significant (P < 0.05) differences in prevalence were observed among herds and according to lactation number. Infections were assigned to four classes, according to the major pathogen, and the respective mean somatic cell counts during the dry season were: S. aureus, 775 x 10(3) cells/ml; CNS, 447 x 10(3) cells/ml; environmental microorganisms, 407 x 10(3) cells/ml; and non-infected, 262 x 10(3) cells/ml. Most of the tested strains were sensitive to antibiotics, and selected strains of S. aureus (n = 15) were negative to the multiplex PCR tests for production of enterotoxins.
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http://dx.doi.org/10.1007/s11250-008-9257-8 | DOI Listing |
Vet Sci
December 2024
Department of Veterinary Medicine and Animal Productions, University of Study of Napoli Federico II, Via Federico Delpino 1, 80137 Napoli, Italy.
The clinical effects on the udder health of several trace elements-copper, iodine, cobalt, and selenium-contained in an intraruminal slow-release bolus were explored for the first time. Fifty-four dairy cows received the bolus (treated group, TG), while fifty-three were left untreated (control group, CG). Monthly composite milk samples were collected from 30 to 300 days in milk to measure somatic cell count (SCC); milk production was also recorded on the same days.
View Article and Find Full Text PDFSci Total Environ
January 2025
Centro Oceanográfico de Vigo, Instituto Español de Oceanografía (IEO-CSIC), Vigo, Spain.
Talanta
February 2025
School of Marine Science and Technology, Harbin Institute of Technology (Weihai), Weihai 264209, China. Electronic address:
A detection method, named as nuclease protection-nucleic acid chromatography strip assay (NP-NACSA), was established to detect Prorocentrum minimum, a common harmful algal blooms-forming algal species. In this study, the developed NP-NACSA requires no nucleic acid amplification, but directly targets rRNA obtained from broken cells. After converting the target rRNA to DNA by nuclease protection, the resultant DNA is automatically analyzed by nucleic acid chromatography strip.
View Article and Find Full Text PDFEnviron Health (Wash)
April 2024
Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention, Department of Environmental Science & Engineering, Fudan University, Shanghai 200433, China.
Bioaerosols pose a great threat to human life and health, and developing highly efficient and accurate identification and analysis methodologies for bioaerosols provides prerequisite knowledge to evaluate their toxicity on human health. In this paper, a rapid and economical method is proposed for the detection of bioaerosols based on surface-enhanced Raman scattering (SERS). The SERS substrate was prepared using an anodic aluminum oxide (AAO) template with an inverted conical (V-shaped) nanopore array structure that was subsequently deposited with Ag nanoparticles (AgNPs) through magnetron sputtering.
View Article and Find Full Text PDFFoods
September 2024
A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, Russia.
and are relevant foodborne bacterial pathogens which may cause serious intoxications and infectious diseases in humans. In this study, a sensitive immunochromatographic analysis (ICA) for the simultaneous detection of these two pathogens was developed. For this, test strips containing two test zones with specific monoclonal antibodies (MAb) against lipopolysaccharides of and and one control zone with secondary antibodies were designed, and the double-assay conditions were optimized to ensure high analytical parameters.
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