Previous studies have shown that a soluble protein fraction derived from preparations of apical plasma membrane (APM) of the oviductal epithelium enhances the in vitro survival of mammalian spermatozoa. Here, we show that the survival enhancing property of the soluble protein fraction seems to depend significantly upon heat shock 70 kDa protein 8 (HSPA8 previously known as HSPA10). The following findings in the present study enabled us to draw this conclusion: first, using proteomic analysis, we identified a subset of 70 kDa oviductal surface proteins that bound to spermatozoa, one of which was HSPA8. Second, pre-treatment of the soluble protein fraction with anti-HSPA8 antibody reduced the 24 h (at 39 degrees C) sperm survival enhancement effect normally induced by the presence of 200 microg/ml soluble APM proteins. Third, complementary experiments showed that substituting the soluble protein fraction with bovine recombinant HSPA8 (0.5-2 microg/ml) also elicited the sperm survival effect. Finally, we also tested the effect of bovine recombinant HSPA8 on bull spermatozoa and found similar, dose-responsive, sperm survival promoting effects. The conserved nature of HSPA8 between mammalian species suggests that this protein may represent a common biological mechanism for the maintenance of sperm survival in the oviduct.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1530/REP-08-0298 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Exogenous melatonin and grafting improved the growth and physiological characteristics of Citrullus lanatus seedlings under cadmium stress.
Environ Sci Pollut Res Int
January 2025
College of Horticulture and Landscape Architecture, Tianjin Agricultural University, Tianjin, 300392, China.
Citrullus lanatus is an important vegetable crop, but it is heavily polluted by cadmium. In this study, we used C. Lanatus as experimental material to investigate effects of different concentrations (0, 50, 100, 200, 400 µmolL) of exogenous melatonin, and grafting on the physiological growth index and anatomical structure of seedlings were studied by simulating Cd (180 mg L) stress environment.
View Article and Find Full Text PDFExploring the Future of Extrusion with Andean Grains: Macromolecular Changes, Innovations, Future Trends and Food Security.
Plant Foods Hum Nutr
January 2025
Facultad de Industrias Alimentarias, Universidad Nacional Agraria La Molina, Lima, Peru.
This review aimed to explore the impact of extrusion on Andean grains, such as quinoa, kañiwa, and kiwicha, highlighting their macromolecular transformations, technological innovations, and contributions to food security. These grains, which are rich in starch, high-quality proteins, and antioxidant compounds, are versatile raw materials for extrusion, a continuous and efficient process that combines high temperatures and pressures to transform structural and chemical components. Extrusion improves the digestibility of proteins and starches, encourages the formation of amylose-lipid complexes, and increases the solubility of dietary fiber.
View Article and Find Full Text PDFAnalysis of IL10 gene promoter haplotypes and changes in mRNA expression and soluble levels in patients with basal cell carcinoma.
Arch Dermatol Res
January 2025
Instituto de Investigación en Ciencias Biomédicas (IICB), Centro Universitario de Ciencias de La Salud, Universidad de Guadalajara, 44340, Guadalajara, Mexico.
Interleukin-10 (IL-10) is an immunomodulatory molecule that may play an immunosuppressive role in nonmelanoma skin cancer (NMSC), specifically basal cell carcinoma (BCC). We analyzed the role of IL10 promoter variants in genetic determinants of BCC susceptibility and their association with IL10 mRNA and IL-10 serum levels. Three promoter variants (- 1082 A > G, - 819 T > C, and - 592 A > C) were examined in 250 BCC patients and 250 reference group (RG) individuals.
View Article and Find Full Text PDFMembrane Proteins in Nanodiscs: Methods and Applications.
ChemMedChem
January 2025
Nankai University, State Key Laboratory of Elemento-Organic Chemistry and Department of Chemical Biology, College of Chemistry, 94 Weijin Road, 300071, Tianjin, CHINA.
Membrane proteins, a principal class of drug targets, play indispensable roles in various biological processes and are closely associated with essential life functions. Their study, however, is complicated by their low solubility in aqueous environments and distinctive structural characteristics, necessitating a suitable native-like environment for molecular analysis. Nanodisc technology has revolutionized this field, providing biochemists with a powerful tool to stabilize membrane proteins and significantly enhance their research possibilities.
View Article and Find Full Text PDF[Expression of soluble factor-related apoptosis ligand in peripheral blood and microRNA-147b in monocytes in children with sepsis and their association with prognosis].
Zhongguo Dang Dai Er Ke Za Zhi
January 2025
PICU, Huai'an Maternal and Child Health Hospital Affiliated to Yangzhou University, Huai'an, Jiangsu 223002, China.
Objectives: To investigate the expression of soluble factor-related apoptosis ligand (sFasL) in peripheral blood and microRNA-147b (miR-147b) in monocytes in children with sepsis and their value in assessing prognosis.
Methods: A prospective study was conducted on 124 children with sepsis (sepsis group), 60 children with common infections (infection group), and 60 healthy children undergoing physical examinations (healthy control group). The independent risk factors for poor prognosis in children with sepsis were analyzed, and the value of serum sFasL and monocyte miR-147b in predicting poor prognosis in children with sepsis was assessed.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!