Objectives: To evaluate the gel permeation high-performance liquid chromatography (GP-HPLC) method for determination of triglyceride (TG) levels in low-density lipoprotein (LDL) and high-density lipoprotein (HDL).
Design And Methods: The GP-HPLC and the ultracentrifugation (UC)/precipitation methods were used and compared.
Results: There was no significant difference in measured levels of LDL-triglyceride between the two methods, but the HDL-triglyceride levels measured by the GP-HPLC technique were significantly higher than the UC/precipitation one (145+/-47 mg/L and 121+/-45 mg/L respectively, n=38, p<0.0001).
Conclusions: A GP-HPLC technique provides LDL-TG levels comparable to those obtained by the UC/precipitation method.
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| http://dx.doi.org/10.1016/j.clinbiochem.2008.10.007 | DOI Listing |
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