Purpose: The bacilli which were isolated from a patient suspected of the mixed infections with Mycobacterium avium and Mycobacterium intracellulare, were analyzed. The genotypes of M. avium in the sedimented fractions of treated sputum and in some colonies isolated from Ogawa medium were compared by the Variable Numbers of Tandem Repeats (VNTR).
Case: A woman, aged 57. Mycobacterial species isolated from some colonies by culture in 2004 and 2006 and from the treated sputum in 2006, were determined by DNA sequencing analysis of the 16S rRNA gene. Also, by using VNTR, the genotype of mycobacteria was analyzed. [Results] (1) The colony isolated from Ogawa medium in 2004 was monoclonal M. avium. (2) By VNTR analyses of specimens in 2006, multiple acid-fast bacteria were found in the sputum sediment and in isolated bacteria from Ogawa medium. (3) By analyses of 16S rRNA DNA sequence, M. avium and M. intracellulare were found in the colonies isolated from the sputum sediment and the Ogawa medium in 2006. (4) The same VNTR patterns were obtained in M. avium in 2004 and 2006 when single colony was analyzed. (5) From the showerhead and culvert of the bathroom in the patient's house, M. avium was not detected.
Discussion: By VNTR analyses, it was considered that the mixed infections of M. avium and M. intracellulare had been generated during treatment in this case. Therefore, in the case of suspected complex infection, VNTR analysis would be a useful genotyping method in M. avium complex infection.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Advancing osseointegration research: A dynamic three-dimensional (3D) culture model for dental implants.
J Dent Sci
January 2025
Weintraub Center for Reconstructive Biotechnology, UCLA School of Dentistry, Los Angeles, CA, USA.
Background/purpose: studies are essential for understanding cellular responses, but traditional culture systems often neglect the three-dimensional (3D) structure of real implants, leading to limitations in cellular recruitment and behavior largely governed by gravity. The objective of this study was to pioneer a novel 3D dynamic osteoblastic culture system for assessing the biological capabilities of dental implants in a more clinically and physiologically relevant manner.
Materials And Methods: Rat bone marrow-derived osteoblasts were cultured in a 24-well dish with a vertically positioned dental implant.
Development of the membrane ceiling method for in vitro spermatogenesis.
Sci Rep
January 2025
Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
Spermatogenesis is one of the most complex processes of cell differentiation and its failure is a major cause of male infertility. Therefore, a proper model that recapitulates spermatogenesis in vitro has been long sought out for basic and clinical research. Testis organ culture using the gas-liquid interphase method has been shown to support spermatogenesis in mice and rats.
View Article and Find Full Text PDFShort Fixation Using Upward/Downward Penetrating Endplate Screws and Percutaneous Vertebral Augmentation for Unstable Osteoporotic Vertebral Fractures.
Spine Surg Relat Res
November 2024
Department of Orthopaedic Surgery, University of Tsukuba, Tsukuba, Japan.
Introduction: Percutaneous vertebral augmentation techniques, such as balloon kyphoplasty (BKP) and vertebral body stenting (VBS), are commonly used for surgical intervention in osteoporotic vertebral fractures (OVFs). However, markedly unstable OVF cases require additional fixation procedures, prompting the exploration of combined percutaneous vertebral augmentation and posterior fixation. A novel surgical approach involving percutaneous vertebral augmentation with upward penetrating endplate screws (PES) and downward PES, complemented by a short fusion of one above one below, was developed.
View Article and Find Full Text PDFChondroprotective functions of neutrophil-derived extracellular vesicles by promoting the production of secreted frizzled-related protein 5 in cartilage.
Cell Commun Signal
November 2024
Department of Orthopedic Surgery, Faculty of Medicine, Graduate School of Medicine, Hokkaido University, Kita-15, Nish-7, Kita-ku, Sapporo, 060-8638, Japan.
Article Synopsis
- Osteoarthritis (OA) is the most prevalent joint disease, involving cartilage breakdown and inflammation, with recent findings indicating that neutrophil extracellular vesicles (EVs) can help reduce inflammation and support cartilage health.
- Research involved isolating these EVs from neutrophils under different conditions to analyze their effects on chondrocytes (cartilage cells) and the underlying molecular mechanisms through mouse models of OA.
- The study revealed that EVs from neutrophils stimulated with TGF-β significantly inhibit harmful factors in chondrocytes, with the protein SFRP5 identified as a key player in these protective effects, suggesting potential avenues for OA treatment.
Soluble form of Lingo2, an autism spectrum disorder-associated molecule, functions as an excitatory synapse organizer in neurons.
Transl Psychiatry
October 2024
Laboratory of Neuropathology and Neuroscience, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan.
Article Synopsis
- * Research identified that levels of Lingo2, a protein linked to excitatory synapse formation, were increased in neurons from the VPA model, suggesting its role as an excitatory synapse organizer.
- * The secreted form of Lingo2 (sLingo2) was found to enhance excitatory synapses in both mouse and human neurons, indicating that better understanding sLingo2's mechanisms could shed light on the development of ASD.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!