There is concern that exposure to bovine thrombin can result in the development of antibodies, usually against factor V/Va, which can lead to hemostatic abnormalities. It is thought that purer preparations of bovine thrombin might be less immunogenic. Utilizing newer methods including a membrane filtration step, bovine crude thrombin is further purified into thrombin 4A and 4B preparations which exhibit a higher specific activity and are devoid of some of the protein contaminants. Bovine crude thrombin and its purified versions were administered intravenously to individual groups of rabbits using standard immunologic protocols. Antiserum was drawn from each rabbit and the pooled antisera were purified to obtain the IgGs using protein G affinity columns. The results suggest that the reported purification process, including filtration, resulted in the removal of most of the antigens found in crude thrombin, and that none of these preparations generated any detectable antibodies against bovine factor V related antigens.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1177/1076029608322550 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Characterization of the mechanisms governing heparin-bovine serum albumin complex interactions and associated anticoagulant activity.
Carbohydr Polym
January 2025
Department of Pharmacy, Jining Medical University, Rizhao, Shandong, China. Electronic address:
Article Synopsis
- * The research found that enoxaparin sodium has a strong binding affinity to BSA, leading to improved anticoagulant activity at a specific concentration, as measured through various coagulation tests.
- * Advanced techniques revealed that the binding between heparin and BSA involved non-covalent interactions, suggesting important structural changes in BSA, which could inform the development of better anticoagulant therapies.
Identification of an osteopontin structural element for the restoration of a normal endometrial epidermal growth factor (EGF) profile determined by the EGF concentration on day 3 of estrous cycle and pregnancy outcome in repeat breeder dairy cows.
Theriogenology
January 2025
Laboratory of Theriogenology, Department of Clinical Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, 060-0818, Japan. Electronic address:
The loss of a cyclic change with two peaks of increased endometrial epidermal growth factor (EGF) concentration on days 2-4 and 13-14 during the estrous cycle has been linked to low fertility in repeat breeder (RB) cows. We have shown that an intravaginal infusion of osteopontin (OPN) restored the EGF profile in RB cows. The present study aimed to determine a structural element of OPN to restore the normal EGF profile and fertility.
View Article and Find Full Text PDFIn vivo thrombin activity in the diatom Phaeodactylum tricornutum: biotechnological insights.
Appl Microbiol Biotechnol
October 2024
Department of Chemistry, Biochemistry and Physics, Université du Québec À Trois-Rivières, Trois-Rivières, QC, Canada.
Diatoms are responsible for 20% of global carbon dioxide fixation and have significant potential in various biotechnological and industrial applications. Recently, the pennate diatom Phaeodactylum tricornutum has emerged as a prominent platform organism for metabolic engineering and synthetic biology. The availability of its genome sequence has facilitated the development of new bioengineering tools.
View Article and Find Full Text PDFErpY-like Protein Interaction with Host Thrombin and Fibrinogen Intervenes the Plasma Coagulation through Extrinsic and Intrinsic Pathways.
ACS Infect Dis
September 2024
Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam 781039, India.
The survival and proliferation of pathogenic within a host are complex phenomena that require careful consideration. The ErpY-like lipoprotein, found on the outer membrane surface of , plays a crucial role in enhancing the bacterium's pathogenicity. The rErpY-like protein, in its recombinant form, contributes significantly to spirochete virulence by interacting with various host factors, including host complement regulators.
View Article and Find Full Text PDFβ-Adrenoceptor Agonists Attenuate Thrombin-Induced Impairment of Human Lung Endothelial Cell Barrier Function and Protect the Lung Vascular Barrier during Resuscitation from Hemorrhagic Shock.
Biomedicines
August 2024
Department of Surgery, University of South Florida Morsani College of Medicine, Tampa, FL 33612, USA.
β-adrenoceptor (β-AR) agonists are known to antagonize thrombin-induced impairment (TII) of bovine and ovine lung endothelial barrier function. The effects of adrenoceptor agonists and other vasoactive agents on human lung microvascular endothelial cell (HULEC-5a) barrier function upon thrombin exposure have not been studied. Furthermore, it is unknown whether the in vitro effects of adrenoceptor agonists translate to lung protective effects in vivo.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!