AI Article Synopsis
- The success of cloned embryo development is heavily influenced by the cell cycle stage of donor cells.
- The study explored how serum starvation, cell confluence, and roscovitine treatment affect cell cycle synchronization in goldfish fibroblasts.
- Results indicated that both cell confluence and roscovitine treatment significantly increased the number of cells in the G0/G1 phase compared to serum starvation, with roscovitine showing effective results at various concentrations.
Article Abstract
One of the most important factors determining the success of the development of cloned embryos is the cell cycle stage of the donor cells. We investigated the effects of serum starvation, culturing to confluence and roscovitine treatment on the cell cycle synchronization of goldfish caudal fin-derived fibroblasts by flow cytometric analysis. The results show that culturing the cells to confluence (85.5%) and roscovitine treatment (82.71%) yield a significantly higher percentage of cells arrested in the G0/G1 (P<0.05) phase than serum starvation (62.85%). Different concentrations of roscovitine (5, 10, or 15 microM) induce cell cycle arrest at the G0/G1 phase.
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| http://dx.doi.org/10.1016/j.cellbi.2008.09.015 | DOI Listing |
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