A method was established to determine water content of fresh meat in deep layer based on NIRS diffusion reflectance spectroscopy by using LEDs. The experimental apparatus was established to investigate internal water content with repeated experiments for pork and mince pork; the water concentration and the water content detected by standard method is linear, and the r2 is larger than 0.90. Calculation of coefficient of variability (CV) and intra class correlation (ICC) procedures were applied to assess test-retest reliability of this measuring method. The CV and ICC values were found to be good (CV < or = 5%; ICC = 0.83). The experimental result indicated that the fast non-invasive measurement method for detecting water content in deep layer of fresh meat based on spatially-resolved spectroscopy was feasible.
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