Weissella hellenica SKkimchi3 produces the higher exopolysaccharide (EPS) on sucrose than lactose, glucose, and fructose at pH 5 and 20 degrees C. Sucrose was exclusively used to cultivate SKkimchi3 in all experiments base on the EPS production tests. The molecular mass of EPS, as determined by gel permeation chroma-tography, was 203,000. (1)H and (13)C NMR analysis indicated that the identity of EPS may be a glucan. When EPS, starch, and cellulose was treated with a-amylase, glucoamylase, glucosidase, and cellulase, glucose was produced from starch and cellulose but was not produced from EPS. Based on HPLC analysis, elemental analysis, (1)H and (13)C NMR analysis, and enzymatic hydrolysis tests, EPS was estimated to be a glucan. EPS suspension was not precipitated even by centrifugation at 10,000xg for 60 min, and EPS made the fermented milk and bacterial culture viscous.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s12275-008-0134-y | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Microbial exopolysaccharides: Classification, biosynthetic pathway, industrial extraction and commercial production to unveil its bioprospection: A comprehensive review.
Int J Biol Macromol
January 2025
Centre for Conservation and Utilization of Blue Green Algae, Division of Microbiology, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India. Electronic address:
Polysaccharides, found universally in all living-species, exhibit diverse biochemical structures and play crucial roles in microorganisms, animals, and plants to defend against pathogens, environmental stress and climate-changing. Microbial exopolysaccharides are essential for cell adhesion and stress resilience and using them has notable advantages over synthetic polysaccharides. Exopolysaccharides have versatile structures and physicochemical properties, used in food systems, therapeutics, cosmetics, agriculture, and polymer industries.
View Article and Find Full Text PDFCharacterization and application of polysaccharides produced by Pseudomonas atacamensis M7D1 for chromium (III) removal from tannery effluent.
Int J Biol Macromol
January 2025
Biotechnology Group, Chemical Engineering Department, Tarbiat Modares University, Tehran, Iran; Modares Environmental Research Institute, Tarbiat Modares University, Tehran, Iran. Electronic address:
The wastewater from various industries contaminated with heavy metals poses significant environmental challenges. Biosorption has emerged as a widely used method for removing heavy metals from industrial wastewater. Pseudomonas atacamensis M7D1 is known to produce polysaccharides, but the potential of its polysaccharides as an adsorbent for heavy metal removal still needs to be explored.
View Article and Find Full Text PDFImmobilization of ammonia oxidizing bacteria using mycelial pellets: Preparation, characteristics, and application for nitritation.
Bioresour Technol
January 2025
College of Urban Construction, Wuhan University of Science and Technology, Wuhan 430065, China.
Ammonia-oxidizing bacteria (AOB) sourced from an aerobic granular sludge (AGS) process were rapidly enriched by progressively increasing ammonia nitrogen (NH-N) loads, achieving a Nitrosomonas abundance of 20.7 % and a nitrite accumulation rate exceeding 80 %. Mycelial pellets formed by Cladosporium, isolated from the same AGS system, provided a porous surface structure for the immobilization of the enriched AOB, creating mycelial pellet/AOB composites.
View Article and Find Full Text PDFDecipher syntrophies and adaptive response towards enhancing conversion of propionate to methane under psychrophilic condition.
Water Res
January 2025
Laboratory of Biomass Bio-chemical Conversion, Guang Zhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China. Electronic address:
Propionate is a key intermediate in anaerobic digestion (AD) under low operational temperatures, which can destabilize the process. In this study, the supplementation of syntrophic cold-tolerant consortia and trace elements significantly improved the performance of psychrophilic (20 °C) reactor, increasing methane production to 91 % of mesophilic reactor levels and reducing propionate concentrations to less than 2 % of those in untreated psychrophilic reactors. Multi-omics analyses revealed that psychrophilic conditions downregulated the methylmalonyl-CoA and aceticlastic methanogenesis pathways.
View Article and Find Full Text PDFModeling aerobic granules in continuously flowing wastewater-treatment processes.
Water Environ Res
January 2025
Arizona State University, Tempe, Arizona, USA.
Continuously flowing wastewater-treatment processes can be configured for biological and physical selection to form and retain large biological aggregates (LBAs), along with suspended biomass that contains ordinary biological flocs and biomass that has detached from the LBAs. Suspended biomass and LBAs have different solids residence times (SRTs) and mass-transport resistances. Here, mathematical sub-models that describe metabolic processes, a 1-D biofilm, and spherical carriers that can migrate throughout a wastewater-treatment process were combined to simulate a full-scale demonstration train having anaerobic, anoxic, and oxic zones, as well as side-stream enhanced biological phosphorus removal.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!