Objective: To establish a convenient, practical and environmental method for extracting and isolating shikimic acid.
Methods: The content of shikimic acid was measured by RP-ion-pair HPLC, the effects of different pH, temperature, sample concentration and eluate concentration on 717 anion exchange resin were studied.
Results: The yield of shikimic acid was 96.52% on the condition of 22 degrees C, pH > 6.5, 7.5 mg /ml sample concentration and 0.03 mol/L HCl eluting.
Conclusion: This method is feasible and suitable for the extraction and isolation of shikimic acid.
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