A sensitive method for determination of ephedrine derivatives using headspace solid-phase microextraction (SPME) with a novel fiber followed by capillary electrophoresis has been developed. The co-poly(butyl methacrylate/hydroxy-terminated silicone oil) (BMA/OH-TSO) was used as stationary phases with the aid of gamma-methacryloxypropyltrimethoxysilane (KH-570) as bridge in SPME using sol-gel-coating method and cross-linking technology. It has high extraction efficiency for ephedrine derivatives in comparison with commercial poly(dimethylsiloxane) and poly(acrylate)-coated fiber. The coating exhibits good thermal and solvent stability as well as long lifetime. A simple and flexible device for desorption of analytes after headspace SPME was constructed. The effect of various experimental parameters for SPME (temperature, time, pH, ionic strength, desorption solvent, etc.) were discussed. Field amplified sample injection (FASI) was applied for on-line sample concentration and a sensitivity enhancement of two orders of magnitude was achieved. Linear ranges were found to be 20-5000 ng/ml. The detection limits for (1R,2S)-ephedrine, (1R,2R)-pseudoephedrine and (1S,2S)-pseudoephedrine were 3, 5 and 5ng/ml, respectively. Relative standard deviation (n=6) was found to be 4.96-7.57%. The method was successfully applied to the analysis of ephedrine derivatives in human urine.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.talanta.2005.06.062 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Colloidal Particle in Suspensions of Maobushisaishinto Extract Granules Enhances Drug Intestinal Penetration.
Chem Pharm Bull (Tokyo)
December 2024
Faculty of Pharmacy, Kindai University.
The main ingredients of Maobushisaishinto (MBST) are ephedrine (EP), methyl eugenol (ME), and aconitine (AC). The pharmacological effects are presumed to be due to the combined effects of these ingredients. In this study, we investigated the impact of the particles present in MBST suspensions on the absorption of the ingredients.
View Article and Find Full Text PDFDesign-assisted HPLC-UV method for therapeutic drug monitoring of pholcodine, ephedrine, and guaifenesin in biological fluids.
Sci Rep
November 2024
Department of Pharmaceutical Analytical Chemistry Faculty of Pharmacy, Mansoura University, 35516, Mansoura, Egypt.
Drug-drug interactions may amplify or diminish their intended effects, or even produce entirely new effects. Multicomponent mixture HPLC analysis offers a thorough and effective method for comprehending the makeup and behavior of complicated materials, advancing research and development across a range of scientific and industrial domains. A novel experimental design-assisted HPLC methodology for the concurrent investigation of the drug-drug interaction of pholcodine, ephedrine, and guaifenesin in biological fluids has been established.
View Article and Find Full Text PDFHarnessing spectrophotometry resolution power for determining ternary mixture for respiratory disorders treatment in their pharmaceutical formulation.
PLoS One
October 2024
Faculty of Pharmacy, Pharmaceutical Analytical Chemistry Department, Beni-Suef University, Alshaheed Shehata Ahmad Hegazy St., Beni-Suef, Egypt.
A ternary mixture incorporating Hydroxyzine hydrochloride (HYX), Ephedrine hydrochloride (EPH) and Theophylline (THP) frequently prescribed for the treatment of respiratory diseases. Herein, two spectrophotometric methods are designated and applied to resolve these three components in their mixture. Method A is ratio-subtraction combined with derivative spectrophotometry, where THP can be determined directly at its λmax 271 nm (neither HYX or EPH interfere), then for determination of HYX and EPH, the ternary mixture was divided by 22 μg/mL of THP and after subtraction of the plateau region, HYX can be determined directly at its λmax 234.
View Article and Find Full Text PDFNatural Binary Herbal Small Molecules Self-Assembled Nanogel for Synergistic Inhibition of Respiratory Syncytial Virus.
ACS Biomater Sci Eng
October 2024
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.
Article Synopsis
- Respiratory syncytial virus (RSV) is a major illness in children with no effective treatments available, prompting research into alternative antiviral therapies.
- The study investigates a new nanomaterial, EPH-GA nanogel, combining glycyrrhizic acid (GA) from licorice and ephedrine (EPH), which enhances antiviral effects against RSV while providing a 100% drug loading efficiency.
- Administering the EPH-GA nanogel to mice showed reduced viral load, improved lung health, and increased bioavailability of EPH, indicating a potential for effective RSV treatment.
A randomized placebo controlled trial demonstrates the effect of dl-methylephedrine on brain functions is weaker than that of pseudoephedrine.
Sci Rep
September 2024
Department of Neuropsychiatry, Graduate School of Medicine, Nippon Medical School, 1-1-5, Sendagi, Bunkyo-ku, Tokyo, 113-8603, Japan.
Intellectual drug doping in athletics by using stimulants that affect central nervous system functions has been diversified. Stimulants are regulated by the World Anti-Doping Agency according to their levels of urinary concentration. Positron emission tomography could evaluate how stimulants affect central nervous system functions.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!