New coulometric method for estimation of blood and plasma total antioxidant capacity (TAC) based on using electrogenerated bromine was proposed. TAC of blood from patients with chronic renal disease undergoing long-term hemodialysis was investigated. Statistical significant changes in TAC level of venous and arterial blood were found. Catalase activity and low density lipoproteins (LDL) concentrations were determined. Linear correlation between TAC and parameters mentioned was found. Contribution from some individual antioxidants was investigated. The developed method for TAC assay is expressive, simple, stable and reliable, and successfully could be used for TAC determination of some biological fluids. This method could be applied in clinic for estimation of blood TAC from patients.
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