Enantioanalysis of l-2-hydroxyglutaric acid in urine samples using enantioselective, potentiometric membrane electrodes based on maltodextrins.

Quantitative assay of l-2-hydroxyglutaric acid (l-2-HGA) is important for the diagnosis of l-2-hydroxyglutaric aciduria. Three enantioselective, potentiometric membrane electrodes (EPMEs) based on maltodextrins with different dextrose equivalent (DE) (DE: 4.0-7.0 (I), 13.0-17.0 (II), 16.5-19.5 (III)), were designed for the enantioanalysis of l-2-HGA. The enantioselective, potentiometric membrane electrodes can be used reliably for enantiopurity assay of l-2-HGA using the direct potentiometric method in the ranges of 10(-9)-10(-5), 10(-6)-10(-3) and 10(-8)-10(-5)molL(-1) for the enantioselective, potentiometric membrane electrodes based on maltodextrins I, II and III, respectively, with very low detection limits. A high reliability was obtained when the electrodes were used for the assay of l-2-HGA in urine samples.