Spectrophotometric determination of gentamycin is described. Gentamycin reacts with p-dimethylaminobenzalhyde in acetic acid/acetate buffer solution to form a Schiff base, a yellow complex, and its maximum absorption wavelength is 405 nm. Effects of pH, kanamycin, streptomycin, beneylpenicillin, lincomycin and foreign ions on the determination of gentamycin have been examined. The absorbance for gentamycin from 0 to 74.40 mug/ml obeys Beer's law. The linear regression equation of the calibration graph is C = 103A - 0.264, with a linear regression correlation coefficient of 0.9997, and recovery from 97 to 101 %. The results obtained by this method agreed with those of the microbiological method. This method is rapid and simple, and can be used for the determination of gentamycin in injection solutions of gentamycin sulphate.
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