An HPLC method for the identification and determination of BHT (3,5-di-tert.butyl-4-hydroxytoluene) and the by-products obtained from it by beta-ray or oxidative treatment is described. The chemical structure of the by-products is established by mass spectrometry. The irradiation leads to the dimeric and trimeric forms of the antioxidant and the oxidation to a cyclohexa-2,5-dien-1-one with substitution of a hydroxy and a methanol or aldehyde group at position 4.
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