A method of determination of (137)Cs in a fission product by extraction with a nitrobenzene solution of lithium dipicrylaminate is described. The effect of pH and the amount of reagent on the efficiency of extraction of micro-amounts of caesium has been studied as well as the competing effect of alkali metal salts; the possibility of substoichiometric determination of caesium has been also investigated. Both procedures have been tested with an artificial mixture of fission products.
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http://dx.doi.org/10.1016/0039-9140(68)80202-4 | DOI Listing |
J Med Chem
January 2025
Louvain Drug Research Institute (LDRI), Medicinal Chemistry Research Group (CMFA), Université Catholique de Louvain (UCLouvain), Brussels B-1200, Belgium.
Arginase-1 (ARG-1) is a promising target for cancer immunotherapy, but the small size and the highly polar nature of its catalytic site present significant challenges for inhibitor development. An alternative strategy to induce enzyme inhibition by targeting protein oligomerization has been developed recently, offering several advantages such as increased selectivity, promotion of protein degradation, and potential substoichiometric inhibition. In this study, we demonstrated that only trimeric ARG-1 is active, which was confirmed by producing monomeric arginase-1.
View Article and Find Full Text PDFACS Appl Mater Interfaces
November 2024
Key Laboratory of Beam Technology of the Ministry of Education, School of Physics and Astronomy, Beijing Normal University, Beijing 100875, China.
Elife
October 2024
Intercollegiate Faculty of Biotechnology of University of Gdańsk and Medical University of Gdańsk, University of Gdańsk, Gdańsk, Poland.
Hsp70 is a key cellular system counteracting protein misfolding and aggregation, associated with stress, ageing, and disease. Hsp70 solubilises aggregates and aids protein refolding through substrate binding and release cycles regulated by co-chaperones: J-domain proteins (JDPs) and nucleotide exchange factors (NEFs). Here, we elucidate the collaborative impact of Hsp110 NEFs and different JDP classes throughout Hsp70-dependent aggregate processing.
View Article and Find Full Text PDFNano Lett
October 2024
Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China.
Elife
August 2024
MRC Prion Unit at UCL, Institute of Prion Diseases, London, United Kingdom.
Prions replicate via the autocatalytic conversion of cellular prion protein (PrP) into fibrillar assemblies of misfolded PrP. While this process has been extensively studied in vivo and in vitro, non-physiological reaction conditions of fibril formation in vitro have precluded the identification and mechanistic analysis of cellular proteins, which may alter PrP self-assembly and prion replication. Here, we have developed a fibril formation assay for recombinant murine and human PrP (23-231) under near-native conditions (NAA) to study the effect of cellular proteins, which may be risk factors or potential therapeutic targets in prion disease.
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