Characterization of new fluorescent labels for ultra-high resolution microscopy.

Photochem Photobiol Sci

Experimental Biomolecular Physics, Department of Applied Physics, Royal Institute of Technology, Stockholm, Sweden.

Published: November 2008

Photo-induced switching of dyes into dark, long-lived states, such as a triplet state, has recently gained increasing interest, as a means to achieve ultra-high optical resolution. Additionally, these long lived states are often highly environment-sensitive and their photodynamics can thus offer additional independent fluorescence-based information. However, although providing a useful mechanism for photo-induced switching, the triplet state often appears as a precursor state for photobleaching, which potentially can limit its usefulness. In this work, a set of rhodamine and pyronin dyes, modified by substitution of heavy atoms and nitrogen within or close to the central xanthene unit of the dyes, were investigated with respect to their triplet state dynamics and photostabilities, under conditions relevant for ultra-high resolution microscopy. Out of the dyes investigated, in particular the rhodamine and pyronin dyes with a sulfur atom replacing the central oxygen atom in the xanthene unit were found to meet the requirements for ultra-high resolution microscopy, combining a prominent triplet state yield with reasonable photostability.

Download full-text PDF

Source
http://dx.doi.org/10.1039/b810991pDOI Listing

Publication Analysis

Top Keywords

triplet state
16
ultra-high resolution
12
resolution microscopy
12
photo-induced switching
8
rhodamine pyronin
8
pyronin dyes
8
xanthene unit
8
dyes investigated
8
dyes
5
state
5

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!