Ghrelin regulates bone formation and osteoblast proliferation, but the detailed signaling pathway for its action on osteoblasts remains unclear. In human osteoblastic TE85 cells, we observed the effects and intracellular signaling pathway of ghrelin on cell proliferation using BrdU incorporation method. Ghrelin, at 10(-10)-10(-8) M concentration, significantly increased BrdU incorporation into TE85 cells. The action of ghrelin was inhibited by D: -Lys3-GHRP-6, a selective antagonist of GHS-R. Nitric oxide (NO) scavenger hemoglobin and the NO synthase inhibitor NAME eliminated the stimulatory action of ghrelin on proliferation, while NO donor SNAP and NO synthase substrate L-AME stimulated proliferation of osteoblastic TE85 cells. The cGMP analogue, 8-Br-cGMP, stimulated TE85 cell proliferation, and ghrelin did not enhance proliferation in the presence of 8-Br-cGMP. Inhibition of cGMP production by the guanylate cyclase inhibitor prevented ghrelin-induced osteoblastic TE85 cell proliferation. In conclusion, ghrelin stimulates proliferation of human osteoblastic TE85 cells via intracellular NO/cGMP signaling pathway.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s12020-008-9117-3 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Development of a 3D Tissue-Engineered Skeletal Muscle and Bone Co-culture System.
Biotechnol J
January 2020
School of Sport, Exercise and Health Sciences, Loughborough University, Loughborough, UK.
In vitro 3D tissue-engineered (TE) structures have been shown to better represent in vivo tissue morphology and biochemical pathways than monolayer culture, and are less ethically questionable than animal models. However, to create systems with even greater relevance, multiple integrated tissue systems should be recreated in vitro. In the present study, the effects and conditions most suitable for the co-culture of TE skeletal muscle and bone are investigated.
View Article and Find Full Text PDFSIRT1 enzymatically potentiates 1,25-dihydroxyvitamin D signaling via vitamin D receptor deacetylation.
J Steroid Biochem Mol Biol
September 2017
Arizona State University, School of Mathematical and Natural Sciences, 4701 W. Thunderbird Road Glendale, AZ 85306, USA; University of Arizona College of Medicine-Phoenix, Department of Basic Medical Sciences, 425 N. 5th Street Phoenix, AZ 85004, USA; University of Arizona Cancer Center,1501 N. Campbell Avenue, Tucson, AZ 85719, USA. Electronic address:
The hormonal metabolite of vitamin D, 1,25-dihydroxyvitamin D (1,25D), binds to the vitamin D receptor (VDR) and promotes heterodimerization of VDR with a retinoid-X-receptor (RXR) to genomically regulate diverse cellular processes. Herein, it is revealed for the first time that VDR is post-translationally acetylated, and that VDR immunoprecipitated from human embryonic kidney (HEK293) cells displays a dramatic decrease in acetylated receptor in the presence of 1,25D-ligand, sirtuin-1 (SIRT1) deacetylase, or the resveratrol activator of SIRT1. To elucidate the functional significance of VDR deacetylation, vitamin-d-responsive-element (VDRE)-based transcriptional assays were performed to determine if deacetylase overexpression affects VDR/VDRE-driven transcription.
View Article and Find Full Text PDFThe effect of MeSO overexposure during cryopreservation on HOS TE85 and hMSC viability, growth and quality.
Cryobiology
December 2016
Centre for Biological Engineering, Department of Chemical Engineering, Loughborough University, Leicestershire, LE11 3TU, UK. Electronic address:
With the cell therapy industry continuing to grow, the ability to preserve clinical grade cells, including mesenchymal stem cells (MSCs), whilst retaining cell viability and function remains critical for the generation of off-the-shelf therapies. Cryopreservation of MSCs, using slow freezing, is an established process at lab scale. However, the cytotoxicity of cryoprotectants, like MeSO, raises questions about the impact of prolonged cell exposure to cryoprotectant at temperatures >0 °C during processing of large cell batches for allogenic therapies prior to rapid cooling in a controlled rate freezer or in the clinic prior to administration.
View Article and Find Full Text PDFc-Fos induction by gut hormones and extracellular ATP in osteoblastic-like cell lines.
Purinergic Signal
December 2016
Musculoskeletal Biology Department, Apex Building, 6 West Derby Street, Liverpool, L7 9TX, UK.
It is widely accepted that the c-Fos gene has a role in proliferation and differentiation of bone cells. ATP-induced c-Fos activation is relevant to bone homeostasis, because nucleotides that are present in the environment of bone cells can contribute to autocrine/paracrine signalling. Gut hormones have previously been shown to have an effect on bone metabolism.
View Article and Find Full Text PDFThe transient receptor potential ion channel TRPV6 is expressed at low levels in osteoblasts and has little role in osteoblast calcium uptake.
PLoS One
May 2012
Department of Human Metabolism, The Mellanby Centre for Bone Research, The University of Sheffield, Sheffield, United Kingdom.
Background: TRPV6 ion channels are key mediators of regulated transepithelial absorption of Ca2+ within the small intestine. Trpv6-/- mice were reported to have lower bone density than wild-type littermates and significant disturbances in calcium homeostasis that suggested a role for TRPV6 in osteoblasts during bone formation and mineralization. TRPV6 and molecules related to transepithelial Ca2+ transport have been reported to be expressed at high levels in human and mouse osteoblasts.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!