AI Article Synopsis
- The study investigates why the JFH-1 strain of Hepatitis C Virus (HCV) can only be efficiently produced in the Huh7 cell line and its variations.
- Researchers compared HCV production in several liver-derived cell lines, including Huh7, HepG2, and IMY-N9, and all produced infectious virus that could infect naïve Huh7 cells.
- Analysis showed differences in the viral particles produced, specifically in the ratio of core protein to RNA, highlighting that viruses from different cell lines may have unique characteristics.
Article Abstract
The efficient production of infectious HCV from the JFH-1 strain is restricted to the Huh7 cell line and its derivatives. However, the factors involved in this restriction are unknown. In this study, we examined the production of infectious HCV from other liver-derived cell lines, and characterized the produced viruses. Clones of the Huh7, HepG2, and IMY-N9, harboring the JFH-1 full-genomic replicon, were obtained. The supernatant of each cell clone exhibited infectivity for naïve Huh7. Each infectious supernatant was then characterized by sucrose density gradient. For all of the cell lines, the main peak of the HCV-core protein and RNA exhibited at approximately 1.15g/mL of buoyant density. However, the supernatant from the IMY-N9 differed from that of Huh7 in the ratio of core:RNA at 1.15g/mL and significant peaks were also observed at lower density. The virus particles produced from the different cell lines may have different characteristics.
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| http://dx.doi.org/10.1016/j.bbrc.2008.10.063 | DOI Listing |
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