AI Article Synopsis
- Poly(ADP-ribosyl)ation is a chemical modification of proteins that plays crucial roles in processes like DNA repair, transcription, cell division, and apoptosis.
- PARP-1 and PARP-2 enzymes contribute to maintaining genome integrity, showing both overlapping and unique functions based on studies with deficient models.
- The degradation of poly(ADP-ribose) is managed by PARG, and the chapter discusses lab techniques to detect and analyze both PARPs and PARG.
Article Abstract
Poly(ADP-ribosyl)ation is a posttranslational modification of proteins in higher eukaryotes mediated by poly(ADP-ribose) polymerases (PARPs) that is involved in many physiological processes such as DNA repair, transcription, cell division, and cell death. Biochemical studies together with PARP-1- or PARP-2-deficient cellular and animal models have revealed the redundant but also complementary functions of the two enzymes in the surveillance and maintenance of genome integrity. Poly(ADP-ribose) is degraded by the endo- and exo-glycosidase activities of poly(ADP-ribose) glycohydrolase (PARG). In this chapter, biochemical and immunofluorescence methods are described for detecting and assaying PARPs and PARG.
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| http://dx.doi.org/10.1007/978-1-60327-461-6_15 | DOI Listing |
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