Colony multiplex-tandem PCR for rapid, accurate identification of fungal cultures.

J Clin Microbiol

Centre for Infectious Diseases and Microbiology, Westmead Hospital, Westmead, New South Wales, Australia.

Published: December 2008

We developed a multiplex tandem PCR (MT-PCR) assay for the rapid identification of 16 fungi directly from culture. MT-PCR results were concordant with phenotypic identification for all cultures studied (n = 183). The colony MT-PCR assay was rapid (<2 h), sensitive, and specific in identifying fungal pathogens directly from primary isolation plates.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2593288PMC
http://dx.doi.org/10.1128/JCM.01411-08DOI Listing

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