ABSTRACT Fusarium oxysporum f. sp. canariensis causes Fusarium wilt disease on the Canary Island date palm (Phoenix canariensis). To facilitate disease management, a polymerase chain reaction diagnostic method has been developed to rapidly detect the pathogen. A partial genomic library of F. oxysporum f. sp. canariensis isolate 95-913 was used to identify a DNA sequence diagnostic for a lineage containing all tested isolates of F. oxysporum f. sp. canariensis. Two oligonucleotide primers were designed and used to amplify a 567-bp fragment with F. oxysporum f. sp. canariensis DNAs. DNA from 61 outgroup isolates did not amplify using these primers. Once the primers were shown to amplify a 0.567-kb fragment from DNA of all the F. oxysporum f. sp. canariensis isolates tested, a rapid DNA extraction procedure was developed that led to the correct identification of 98% of the tested F. oxysporum f. sp. canariensis isolates.
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http://dx.doi.org/10.1094/PHYTO.1999.89.5.407 | DOI Listing |
Int J Mol Sci
December 2024
Instituto Universitario de Bio-Orgánica Antonio González, Departamento de Química Orgánica, Universidad de La Laguna, Avenida Astrofísico Francisco Sánchez 2, 38206 La Laguna, Tenerife, Spain.
Considering the detrimental impacts of the current pesticides on the biotic components of the biosphere, the development of novel pesticides is vital. Plant-derived biopesticides have emerged as popular alternatives to create a safer and more sustainable agriculture model. This study aims to validate the previous bioguided fractionation of endemic Canary Islands sage, , as a potential source of botanical pesticides using a cultivation process.
View Article and Find Full Text PDFNat Prod Res
October 2024
Laboratory of Organic Chemistry LR17ES08 (Natural Substances Team), University of Sfax, Faculty of Sciences of Sfax, Sfax, Tunisia.
In this study, a novel fatty acid anhydride, named phalarisin 1, was successfully isolated from the ethyl acetate extract of growing in Tunisia. The structural characterisation of the compound was achieved through various spectroscopic techniques including FT-IR, 1D and 2D-NMR spectroscopy, as well as acidic hydrolysis analyses. Remarkably, phalarisin exhibited potent antimicrobial properties against a range of pathogens including , , , and , with minimum inhibitory concentrations (MICs) ranging from 31.
View Article and Find Full Text PDFPest Manag Sci
April 2024
Departamento de Química Orgánica, Instituto Universitario de Bio-Orgánica Antonio González, Universidad de La Laguna, La Laguna, Spain.
Background: Biofungicides arise as a promising alternative to the indiscriminate use of harmful synthetic fungicides in crop management.
Results: The present study reports the bio-guided fractionation of an endemic plant from the Canary Islands, Salvia canariensis against the phytopathogens, Alternaria alternata, Botrytis cinerea, and Fusarium oxysporum. This procedure allowed identifying a series of diterpenoids with an abietane skeleton (1-5), which exhibited remarkable activity against the phytopathogenic fungi assayed.
Plants (Basel)
November 2022
Instituto Universitario de Bio-Orgánica Antonio González, Departamento de Química Orgánica, Avenida Astrofísico Francisco Sánchez 2, 38206 La Laguna, Tenerife, Spain.
Phytopathogens are responsible for great losses in agriculture. In particular, Fusarium, Alternaria and Botrytis are fungal diseases that affect crops worldwide. In the search for eco-friendly solutions to pest control, plants and their chemo-biodiversity are promising sources of biopesticides for integrated pest management.
View Article and Find Full Text PDFPlant Dis
June 2021
The University of Jordan, Plant Protection, Queen Rania St, Amman, Jordan, 11942;
Forests of Jordan are located in the northern and southern parts of the country with 60% and 40%, respectively. Natural forests constitute about 75% in the northern part and 25% in the southern part. There are many types of forest trees in Jordan including pines (Pinus spp.
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