AI Article Synopsis
- The study investigates the aphid transmission characteristics of a specific isolate of potato virus Y (PVY 1-2), finding that it does not transmit between plants on its own.
- Despite this, the virus can be transmitted when its particles are combined with a helper component (HC) from another transmissible isolate (PVY 0 AT), indicating the coat protein is functional for transmission.
- Genetic analysis revealed 19 nucleotide differences between PVY 1-2 and PVY 0 AT, with only two causing amino acid changes; however, these mutations are not within key areas typically associated with aphid transmission in other potyviruses.
Article Abstract
ABSTRACT The aphid transmission properties of a pepper isolate of potato virus Y belonging to the pathotype 1-2 (PVY 1-2) have been characterized. PVY 1-2 was not transmitted in plant-to-plant experiments, although purified virus particles were efficiently transmitted when supplemented with heterologous helper component (HC) of the transmissible isolate PVY 0 AT through membrane acquisition assays, indicating that its coat protein was functional in transmission. Additionally, virions of PVY 1-2 were able to bind to different HCs in in vitro binding assays. Analysis of the sequence of the PVY 1-2 HC gene and comparison with that of PVY 0 AT revealed 19 nucleotide differences, but only 2 resulted in amino acid changes, one of which induced a change of charge. Neither of these two amino acid changes occurred within the cysteine-rich domain, nor did they coincide with conserved motifs of the HC protein known to be involved in aphid transmission and which are present in all known potyvi-ruses. However, both changes are located in positions highly conserved among PVY strains. The possible role of both mutations on the activity of the PVY 1-2 HC in aphid transmission is discussed.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1094/PHYTO.1999.89.12.1176 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Discovery of Pyrido[1,2-α] Pyrimidinone Mesoionic Compounds as Potential Control Agents Against Potato Virus Y.
J Agric Food Chem
June 2024
State Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Huaxi, Guiyang 550025, China.
Potato virus Y (PVY) relies on aphids and tubers to spread in the field and causes serious economic losses in the potato industry. Here, we found that pyrido[1,2-α] pyrimidinone mesoionic compounds with insecticidal activity against aphids possessed a good inhibitory effect on PVY. Among them, compound had the best inhibitory activity against PVY (EC = 104 μg/mL), even superior to that of ningnanmycin (125 μg/mL).
View Article and Find Full Text PDFFirst report of Melon chlorotic spot virus in cultivated sorrel () in Belgium.
Plant Dis
December 2023
Gembloux Agro-Bio Tech, University of Liège Gembloux, Plant Pathology Laboratory, Passage des deportes, 2, Gembloux, Belgium, 5030;
In 2020, symptoms of putative viral origin were observed on 7% of tomatoes in an organic vegetable farm in Belgium (deformed uneven ripened fruits, vein clearing, mosaic and purple leaves, stunted plants). The leaves of twenty symptomatic plants were collected, pooled and screened for viruses using high throughput sequencing technologies (HTS) on Illumina NextSeq500 following a virion-associated nucleic acid (VANA) protocol (Temple et al., 2021, Be_SL1).
View Article and Find Full Text PDFDesign, Synthesis, and Anti-PVY Biological Activity of 1,3,5-Triazine Derivatives Containing Piperazine Structure.
Int J Mol Sci
May 2023
Center for R&D of Fine Chemicals of Guizhou University, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, National Key Laboratory of Green Pesticide, Guiyang 550025, China.
In this study, a commercial agent with antivirus activity and moroxydine hydrochloride were employed to perform a lead optimization. A series of 1,3,5-triazine derivatives with piperazine structures were devised and synthesized, and an evaluation of their anti-potato virus Y (PVY) activity revealed that several of the target compounds possessed potent anti-PVY activity. The synthesis of compound was directed by a 3D-quantitative structure-activity relationship that used the compound's structural parameters.
View Article and Find Full Text PDFClasnip: a web-based intraspecies classifier and multi-locus sequence typing for pathogenic microorganisms using fragmented sequences.
PeerJ
January 2023
Charlottetown Laboratory, Canadian Food Inspection Agency, Charlottetown, Prince Edward Island, Canada.
Bioinformatic approaches for the identification of microorganisms have evolved rapidly, but existing methods are time-consuming, complicated or expensive for massive screening of pathogens and their non-pathogenic relatives. Also, bioinformatic classifiers usually lack automatically generated performance statistics for specific databases. To address this problem, we developed Clasnip (www.
View Article and Find Full Text PDFFirst Discovery of Imidazo[1,2-]pyridine Mesoionic Compounds Incorporating a Sulfonamide Moiety as Antiviral Agents.
J Agric Food Chem
June 2022
State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Center for Research and Development of Fine Chemicals, Guizhou University, Guiyang, Guizhou 550025, People's Republic of China.
The applications of mesoionic compounds and their analogues as agents against plant viruses remain unexplored. This was the first evaluation of the antiviral activities of mesoionic compounds on this issue. Our study involved the design and synthesis of a series of novel imidazo[1,2-]pyridine mesoionic compounds containing a sulfonamide moiety and the assessment of their antiviral activities against (PVY).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!