[Dynamics of histamine content in the rat uterus compartments according to the stages of the estrous cycle (a fluorescence histochemical study)].

The purpose of the present study was to detect the main histamine-containing structures of the rat uterus and to study the changes of its content during the estrous cycle. The work was performed during the autumn-winter period using 120 female rats that were divided into groups according to the stages of the estrous cycle: early estrus, late estrus, metestrus, early diestrus, late diestrus, proestrus. Cryostat sections of uterine corpus and cervix were studied using fluorescent-histochemical method with the orthophthalic aldehyde. The histamine content was determined in mast cells, macrophages, surface and glandular epithelial cells, smooth myocytes in the tunics of the uterine corpus and cervix, as well as in the contents of the uterine cavity. During the sexual cycle, the highest histamine concentrations were detected in mast cells, especially in those located in the myometrium. In most of the bioamine-positive cellular elements of the uterine wall, the minimal histamine content was found in metestrus. As a result of a computer-statistical analysis, it was demonstrated that the intraorgan histamine metabolism in the structures of the uterine tunics studied, was characterized by phase and regional specificity together with the dynamic nature of temporal coupling of histamine content.