AI Article Synopsis
- This study presents the first spectroscopic analysis of c-type ions produced by electron capture dissociation (ECD) of a peptide.
- There is ongoing debate about whether these ions have an enol-imine or an amide structure due to their isomeric nature, which makes mass spectrometry alone insufficient for differentiation.
- Using a combination of a free electron laser and FT-ICR mass spectrometry, the researchers determined that c-type ions possess an amide structure, evidenced by a distinct infrared signature at 1731 cm(-1).
Article Abstract
In this study, we propose the first spectroscopic structural characterization of c-type ions produced by ECD of a peptide. The structure of c-type ions formed by electron capture dissociation and the overall mechanism leading to their formation are still a question of debate. Depending on the mechanism, c-type ions have been proposed to have either an enol-imine structure (-C(OH)NH) or an amide one (-C(O)-NH2). Since these ions are isomeric, mass spectrometry only cannot discriminate between them, but infrared spectroscopy can bring experimental evidence and help determine which scheme is operative. Using the coupling between a tunable free electron laser and a FT-ICR mass spectrometer, we show that c-type ions have an amide structure, characterized by an IR signature of the C=O stretch at 1731 cm(-1). This result is particularly interesting from the perspective of the elucidation of the ECD mechanism.
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| http://dx.doi.org/10.1021/ja805257v | DOI Listing |
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