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Improving Septicemia Diagnostics: A Comparative Analysis of Direct and Post-Culture MALDI-TOF MS Methods for Bacterial Identification.
Tunis Med
December 2024
Faculty of Medicine and Pharmacy, Mohammed V University in Rabat, Morocco.
Article Synopsis
- Bloodstream infections need quick bacterial identification for effective treatment, with traditional methods being slow.
- The study compared direct identification using MALDI-TOF MS with the post-culture method, finding that while direct identification had a lower success rate (64.8%), it provided results in under an hour.
- Despite its limitations, the direct method could significantly enhance diagnosis speed, making it a potentially valuable tool in conjunction with established methods.
[PATHOGENETIC ASPECTS OF REHABILITATION OF PATIENTS AFTER CHOLECYSTECTOMY].
Eksp Klin Gastroenterol
June 2016
Unlabelled: Investigation of efficiency of liquid synbiotics and structure-resonance electric magnetic therapy (SRMT) among patients after cholecystectomy.
Materials And Methods: 90 patients after cholecystectomy have been investigated (CE). Along with general clinical meth-ods of investigation, patients passed US investigation of abdomen, biochemical blood tests, bacteriological test of faeces, investigation of short-chain fatty acids (SCFA) by gas-liquid osteal chromatographic analysis.
Diagnosis of pulmonary tuberculosis in a microbiological laboratory.
Med Mal Infect
October 2011
Unité de recherche sur les maladies infectieuses et tropicales émergentes, CNRS UMR 6236, faculté de médecine, 27 boulevard Jean-Moulin, Marseille cedex 5, France.
The positive diagnosis of pulmonary tuberculosis still relies on the direct detection of Mycobacterium tuberculosis complex strains after isolation, culture, and identification, or on the detection of specific DNA sequences. Clinical specimens for the direct diagnosis include respiratory tract and stool specimens, which can all be collected with a "tuberculosis kit" to simplify and standardize clinical and laboratory tasks. Gastric juice and other specimens are no longer useful.
View Article and Find Full Text PDF[Report on fourth national epidemiological sampling survey of tuberculosis].
Zhonghua Jie He He Hu Xi Za Zhi
January 2002
Beijing Tuberculosis and Thoracic Tumour Research Institute, 101149 Beijing, China.
Objective: To investigate the epidemiological trend of tuberculosis, to evaluate the efficacy of control measures and to provide scientific basis for making National Tuberculosis Control Programme 2001 approximately 2010.
Methods: Tuberculin testing was carried out among 0 approximately 14 years old children; fluroscopy was carried out for >/= 15 years old population and children with >/= 10 mm reaction of tuberculin testing; chest X-ray film, sputum smear and culture were done for the patients of fluroscopy abnormal and suspects of tuberculosis symptom (persistent cough for 3 weeks or more); drug sensitivity test was done for the patients with culture positive; a retrospective study of tuberculosis mortality in 1999 was conducted at all investigation points; social economic study was done for the active pulmonary tuberculosis cases; the survey of tuberculosis infection rate for all population was carried out in 59 investigation points.
Results: The population actually examined in this survey numbered 365 097.
[The application of the hybridization in colonies technic for the identification of toxigenic Vibrio cholerae 01].
Rev Cubana Med Trop
November 1998
Instituto de Medicina Tropical Pedro Kourí.
By means of the polymerase chain reaction (PCR) it was obtained a probe for the gen that codifies the subunit B of cholerae toxin (CTxB), which carried a Vibrio cholerae 01 reference strain. The checking of the amplified product was performed by using the hybridization techniques in colonies. This product hybridized with the gen that codifies for the subunit B of cholerae toxin isolated from Peru and Ecuador, representing the present epidemics in Latin America, but it did not so with the phylogenetically related strains.
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