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http://dx.doi.org/10.1016/b978-0-7506-9188-8.50020-6 | DOI Listing |
Viruses
January 2025
Research Center for Life Sciences Computing, Zhejiang Lab, Hangzhou 311100, China.
, a medicinal herbaceous plant documented in the Chinese Pharmacopoeia, is a promising candidate for research into plant-derived pharmaceuticals. However, the study of newly emerging viruses that threaten the cultivation of remains limited. In this study, plants exhibiting symptoms such as leaf yellowing, mottled leaves, and vein chlorosis were collected and subjected to RNA sequencing to identify potential viral pathogens.
View Article and Find Full Text PDFInt J Mol Sci
January 2025
Xinjiang Production and Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, College of Life Science, Tarim University, Alar 843300, China.
This study aims to identify the function of the () gene in the kidneys of . CYP2E1 is a significant metabolic enzyme involved in the metabolism of various endogenous and exogenous compounds and is associated with the occurrence and progression of multiple diseases. Given 's ability to survive in the extremely arid , we hypothesize that CYP2E1 in its kidneys plays a crucial role in adaptability.
View Article and Find Full Text PDFMicroorganisms
January 2025
Institute of Food Science and Biotechnology, Department of Bioprocess Engineering, University of Hohenheim, Fruwirthstraße 12, 70599 Stuttgart, Germany.
Background: Cow's milk represents an important protein source. Here, especially casein proteins are important components, which might be a promising source of alternative protein production by microbial expression systems. Nevertheless, caseins are difficult-to-produce proteins, making heterologous production challenging.
View Article and Find Full Text PDFMicroorganisms
December 2024
Department of Chemical, Biological and Environmental Engineering, Universitat Autònoma de Barcelona, Carrer de les Sitges, s/n, 08193 Bellaterra, Catalonia, Spain.
The introduction of heterologous pathways into microbial cell compartments offers several potential advantages, including increasing enzyme concentrations and reducing competition with native pathways, making this approach attractive for producing complex metabolites like fatty acids and fatty alcohols. However, measuring subcellular concentrations of these metabolites remains technically challenging. Here, we explored 3-hydroxypropionic acid (3-HP), readily quantifiable and sharing the same precursors-acetyl-CoA, NADPH, and ATP-with the above-mentioned products, as a reporter metabolite for peroxisomal engineering in the yeast .
View Article and Find Full Text PDFSheng Wu Gong Cheng Xue Bao
January 2025
Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, College of Bioengineering, Tianjin University of Science & Technology, Tianjin 300457, China.
-methyl-L-cysteine sulfoxide (SMCO) is a non-protein sulfur-containing amino acid with a variety of functions. There are few reports on the enzymes catalyzing the biosynthesis of SMCO from -methyl-L-cysteine (SMC). In this study, the flavin-containing monooxygenase gene derived from () was heterologously expressed in BL21(DE3) and the enzymatic properties of the expressed protein were analyzed.
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