PCR amplification of pure bacterial DNA is vital to the study of bacterial interactions with corals. Commonly used Bacteria-specific primers 8F and 27F paired with the universal primer 1492R amplify both eukaryotic and prokaryotic rRNA genes. An alternative primer set, 63F/1542R, is suggested to resolve this problem.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2607152 | PMC |
| http://dx.doi.org/10.1128/AEM.01303-08 | DOI Listing |
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