Setting: Cervical tuberculous lymphadenitis (CTBL) diagnosis is a critical problem due to the difficulty in culturing Mycobacterium tuberculosis.
Objective: To evaluate a nested polymerase chain reaction (nPCR) for the diagnosis of CTBL.
Design: Thirty-eight children initially diagnosed on the basis of clinical and laboratory criteria as suffering from chronic cervical lymphadenitis were included in the study. Forty-one cervical lymph node specimens were analysed by bacterial staining, culture, cytology or histopathology. The IS6110 DNA sequence of M. tuberculosis complex was amplified by nPCR. Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and efficiency were determined for the assay.
Results: The sensitivity of nPCR was 96%, the specificity 93%, PPV 96%, NPV 93% and efficiency 95%. Among 25 patients with CTBL, six presented a 'definite' diagnosis (24%) according to established criteria; 10 were classified as 'highly probable' cases (40%) and nine presented a 'possible' diagnosis (36%). The sensitivity of nPCR was higher than the sensitivity of staining (15%), culture (26%) and cytology or histopathology (62.5%) (95%CI P < 0.05, chi(2) P < 0.001).
Conclusion: The nPCR used is a highly sensitive, specific and efficient method for the diagnosis of CTBL among children.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Haplotypes of Chloroquine Resistance Marker Genes Among Uncomplicated Malaria Cases in Lagos, Nigeria.
Biochem Genet
January 2025
Key Laboratory of Parasite and Vector Biology of the Chinese Ministry of Health, Chinese Center for Disease Control and Prevention, WHO Collaborating Centre for Tropical Diseases, National Institute of Parasitic Diseases, Shanghai, 200025, People's Republic of China.
Drug resistance resulting from mutations in Plasmodium falciparum, that caused the failure of previously effective malaria drugs, has continued to threaten the global malaria elimination goal. This study describes the profiles of P. falciparum chloroquine resistance transporter (Pfcrt) and P.
View Article and Find Full Text PDFMorphological and molecular characteristics of swine coccidia based on single oocyst isolation.
Parasitol Res
January 2025
College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450046, P.R. China.
Swine coccidiosis is a host-specific protozoan disease caused by Cystoisospora suis and various Eimeria species, leading to diarrhea or subclinical signs in pigs. In this study, 3296 fecal samples from 55 farms across six provinces in China were collected and examined to determine the prevalence and molecular characteristics of swine coccidia. The single oocyst isolation technique (SOIT) and molecular characterization identified nine coccidian species, with an overall infection prevalence of 13.
View Article and Find Full Text PDFGenomic and evolutionary analysis of epidemic porcine hepatitis E virus (HEV) in the Tibetan Plateau was performed. Faecal samples were collected from 216 Tibetan pigs and 78 Tibetan Yorkshire (Large White) and 53 tissue samples from Yorkshire from the Linzhi City slaughterhouse. Total RNA was extracted from faeces and fragments of HEV open reading frame 2 (ORF2) detected by reverse transcription and nested polymerase chain reaction (RT-nPCR) and cloned.
View Article and Find Full Text PDFPrevalence of Site-Specific Infection and Macrolide and Fluoroquinolone-Associated Mutations in Men Who Have Sex with Men in Shenzhen, China.
Infect Drug Resist
January 2025
School of Public Health, Fudan University, Shanghai, People's Republic of China.
Background: (MG) poses a growing public health concern due to the escalating antimicrobial resistance. We aimed to assess site-specific MG infection and its correlates and macrolide and fluoroquinolones mutations among men who have sex with men (MSM) in Shenzhen, China.
Methods: Samples were obtained from different anatomic sites of MSM based on their sexual behavior.
Detection of low frequency artemisinin resistance mutations, C469Y, P553L and A675V, and fixed antifolate resistance mutations in asymptomatic primary school children in Kenya.
BMC Infect Dis
January 2025
Centre for Geographic Medicine Research (Coast), Kenya Medical Research Institute-Wellcome Trust Research Programme, Kilifi, Kenya.
Background: To understand the emergence and spread of drug-resistant parasites in malaria-endemic areas, accurate assessment and monitoring of antimalarial drug resistance markers is critical. Recent advances in next-generation sequencing (NGS) technologies have enabled the tracking of drug-resistant malaria parasites.
Methods: In this study, we used Targeted Amplicon Deep Sequencing (TADS) to characterise the genetic diversity of the Pfk13, Pfdhfr, Pfdhps, and Pfmdr1 genes among primary school-going children in 15 counties in Kenya (Bungoma, Busia, Homa Bay, Migori, Kakamega, Kilifi, Kirinyaga, Kisii, Kisumu, Kwale, Siaya, Tana River, Turkana, Vihiga and West Pokot).
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!