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http://dx.doi.org/10.1155/S1463924686000263 | DOI Listing |
PLoS One
December 2024
Division of Biology, Chemistry, and Materials Science, Office of Science and Engineering Laboratories, Center for Devices and Radiological Health, US Food and Drug Administration (FDA), Silver Spring, MD, United States of America.
During the SARS-CoV-2 pandemic, a need for methods to decontaminate and reuse personal protective equipment (PPE) and medical plastics became a priority. In this investigation we aimed to develop a contamination evaluation protocol for laboratory pipette tips, after decontamination. Decontamination methods tested in this study included cleaning with a common laboratory detergent (2.
View Article and Find Full Text PDFElife
November 2024
George W Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, United States.
Significant technical challenges exist when measuring synaptic connections between neurons in living brain tissue. The patch clamping technique, when used to probe for synaptic connections, is manually laborious and time-consuming. To improve its efficiency, we pursued another approach: instead of retracting all patch clamping electrodes after each recording attempt, we cleaned just one of them and reused it to obtain another recording while maintaining the others.
View Article and Find Full Text PDFAnal Chem
November 2024
Advanced Fuel Cycle Technology Division, Korea Atomic Energy Research Institute, 111 Daedeok-daero 989, Yuseong-gu, Daejeon 34057, Republic of Korea.
We propose a sensor event-driven, open-column chromatographic sample preparation system─OpenPrep. This system replaces the problematic flow selector valve with a noncontact built-in sensor, eliminating carryover and clogging issues, which are commonly encountered in pump-driven chromatographic systems. The innovative valveless column-flow design, combined with a compact gantry dispenser and motion stage-based fraction collector, reduces the sample flow path to a disposable component with a postcolumn dead volume of only 0.
View Article and Find Full Text PDFJ Vet Med Educ
September 2024
Antech Diagnostics, Tucson, AZ 85743, USA.
Being able to prepare a diagnostic blood smear for evaluation is an essential skill for veterinary practitioners. While this basic technique can be demonstrated via videos or illustrations, it can only be learned through practice and with feedback. With the necessity for virtual learning experiences during the COVID-19 pandemic, a model was developed and paired with an online module to facilitate at home instruction and practice of blood smear preparation.
View Article and Find Full Text PDFCells Tissues Organs
October 2024
Department of Pediatric Surgery, Stem Cell Biology and Therapy Laboratory, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing, China.
Introduction: Cellular wound healing assay is an important experimental technique for detecting cell migration in vitro. Scratching on monolayer cells using a pipette tip is commonly used. However, it is difficult to guarantee the scratch with the same width, and the initial scratch width has a large impact on the experimental results for different treatment factors or different cell types.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!