Evaluation of use of the optional unit QA-810V for the determination of five-part leukocyte differentials.

The newly developed QA-810V is an optional unit for the determination of five-part white blood cell differentials. It can be used together with the same manufacturer's haematology analyser which has been used in relatively small-sized laboratories. The present study evaluates the basic performance of the QA-810 V and the MEK-8118 haematology analyser using routinely obtained blood specimens treated with ethylenedioaminetetraacetic acid-2K. In this evaluation, reproducibility was good and little carryover was found. Accurate measurements were possible for up to 24h of storage. Storage at 4 degrees C yielded more stable measurements of complete blood counts and five-part differentials than storage at room temperature. A good correlation between findings with the MEK-8118 haematology analyser and those with the SE-9000 haematology analyser was found for complete blood counts. The leukocyte differential obtained with the QA-810 V correlated well with eye counts, with r > 0.9 for percentages of neutrophils, lymphocytes and eosinophils. Scattergrams obtained with the QA-810 V reflected the presence of abnormal cells. The performance of the QA-810 V was excellent and it can improve the quality of testing in clinical laboratories.