Heterogeneity of four lines derived from a single strain of pseudorabies virus (PRV) was studied by isoelectric separation. The individual lines differed in the distribution pattern of infectious fractions separated in the pH range from 3.5 to 10.0. All virus fractions of the virulent and attenuated lines were infectious, but some fractions of the highly attenuated avirulent line were devoid of infectivity. Infectivity of the fractions isolated from the individual PVR lines depended on the degree of attenuation; the higher degree of attenuation, the greater the differences in infectivity of the isolated fractions. The possible causes of the different distribution of infectious fractions obtained by isoelectric separation from virus lines differing in biological properties are discussed. The relationship between physico-chemical and biological properties of PRV is stressed along with the possibilities of applying isoelectric separation in narrow pH gradients.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
High-level biosynthesis and purification of the antimicrobial peptide Kiadin based on non-chromatographic purification and acid cleavage methods.
Biotechnol Biofuels Bioprod
January 2025
Department of Animal Science and Technology, University of Northwest A&F, Yangling, 712100, Shaanxi, China.
Antimicrobial peptides (AMPs) are renowned for their potent bacteriostatic activity and safety, rendering them invaluable in animal husbandry, food safety, and medicine. Despite their potential, the physiological toxicity of AMPs to host cells significantly hampers their biosynthetic production. This study presents a novel approach for the biosynthesis of the antimicrobial peptide Kiadin by engineering a DAMP4-DPS-Kiadin fusion protein to mitigate host cell toxicity and achieve high-level expression.
View Article and Find Full Text PDFHow peptide migration and fraction bioactivity are modulated by applied electrical current conditions during electromembrane process separation: A comprehensive machine learning-based peptidomic approach.
Food Res Int
January 2025
Department of Food Science, Université Laval, Québec G1V 0A6, Canada; Laboratoire de Transformation Alimentaire et Procédés ÉlectroMembranaires (LTAPEM, Laboratory of Food Processing and ElectroMembrane Processes), Université Laval, Québec G1V 0A6, Canada; Institute of Nutrition and Functional Foods (INAF), Université Laval, Québec G1V 0A6, Canada. Electronic address:
Industrial wastewaters are significant global concerns due to their environmental impact. Yet, protein-rich wastewaters can be valorized by enzymatic hydrolysis to release bioactive peptides. However, achieving selective molecular differentiation and eventually enhancing peptide bioactivities require costly cascades of membranes.
View Article and Find Full Text PDFImaged capillary isoelectric focusing and online mass spectrometry for milk whey protein characterization in dairy products.
Anal Biochem
January 2025
Advanced Electrophoresis Solutions Ltd., 380 Jamieson Parkway, Unit 7 and 8, ON, N3C 4N4, Canada; AES Biotech Jiaxing Ltd., No. 501 South Changsheng Road, Economic and Technological Development Zone, Jiaxing City, Zhejiang Province, PR China. Electronic address:
Characterizing major bovine milk proteins, including whey and casein, is of significant interest in the dairy industry. The diverse array of protein proteoforms can be different in terms of genetic variation, breed ways, lactation stage, and animal nutritional status. Current routine methods for bovine milk protein profiling are typically based on immunological techniques, infrared spectroscopy, slab gel isoelectric focusing, capillary electrophoresis, and high-performance liquid chromatography.
View Article and Find Full Text PDFTechnical considerations regarding saliva sample collection to achieve comparable protein identification and detection via one- and two-dimensional gel electrophoresis among humans.
Heliyon
December 2024
Department of Biochemistry and Medical Chemistry, University of Pecs, Medical School, Pecs, Hungary.
Background And Aims: Recently, demands towards identifying various molecules in support of stress detection and potential clinical utilization are dramatically increasing. Moreover, the accuracy with which researchers quantify these informative molecules is now far more improved when compared to the past. As RNA or protein markers are conventionally detected via repeated invasive procedures from blood, it is critical to develop secure technologies to obtain the desired information via less stressful methodologies, such as saliva collection.
View Article and Find Full Text PDFProtein concentration and analyzing charge variants in a co-formulation comprising three monoclonal antibodies: A cation-exchange chromatography approach.
Int J Pharm
January 2025
BioDev Drug Product Development Department, WuXi Biologics, 190 Hedan Road, Shanghai 200131, China. Electronic address:
In the realm of therapeutic antibodies, co-formulations comprising two or more monoclonal antibodies (mAbs) have emerged as a promising strategy, offering enhanced treatment efficacy, improved efficiency, and prolonged intellectual property protection. These advantages have sparked significant interest among both patients and pharmaceutical companies. However, the quantification and analysis of individual mAbs within such co-formulations pose a substantial challenge due to their similar physicochemical properties.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!