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Sprayed Aqueous Microdroplets for Spontaneous Synthesis of Functional Microgels.
Angew Chem Int Ed Engl
January 2025
DWI-Leibniz-Institute for Interactive Materials, 52074, Aachen, Germany.
The development of sustainable synthesis route to produce functional and bioactive polymer colloids has attracted much attention. Most strategies are based on the polymerization of monomers or crosslinking of prepolymers by enzyme- or cell-mediated reactions or specific catalysts in confined emulsions. Herein, a facile solution spray method was developed for spontaneous synthesis of microgels without use of confined emulsion, additional initiators/catalysts and deoxygenation, which addresses the challenges in traditional microgel synthesis.
View Article and Find Full Text PDFStructure-switchable branched inhibitors regulate the activity of CRISPR-Cas12a for nucleic acid diagnostics.
Anal Chim Acta
January 2025
Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan, People's Republic of China; Wuhan Research Center for Infectious Diseases and Cancer, Chinese Academy of Medical Sciences, Wuhan, People's Republic of China; Hubei Engineering Center for Infectious Disease Prevention, Control and Treatment, Wuhan, People's Republic of China. Electronic address:
Article Synopsis
- CRISPR-based strategies are emerging as powerful tools for gene editing and diagnostics, but current technologies require additional amplification methods, complicating detection workflows.
- A novel technique using enzyme-responsive, steric hindrance-based inhibitors has been developed to enhance CRISPR/Cas12a's nucleic acid detection capabilities by allowing real-time and one-pot detection.
- The proposed method improves sensitivity and programmability in molecular diagnostics and can be applied universally to detect various targets like HIV-1 DNA.
A Pilot Study to Evaluate the Minimally Invasive Burn Care for Small, Deep Partial-Thickness Burns of the Hands and Feet Using Enzyme Debridement and Autologous Skin Cell Spray.
J Clin Med
December 2024
Department of Plastic and Reconstructive Surgery, Tokyo Medical University, Tokyo 160-0023, Japan.
: We treated deep partial-thickness burns of the hands and feet in four cases using a combination of NexoBrid and ReCell autologous cell regeneration techniques, without conventional split-thickness skin graft, with good results following debridement of the eschar. : We report cases of patients treated with a combination of the NexoBrid and ReCell techniques between 1 August 2023 and 31 July 2024. The degree of debridement and the time to complete wound closure were evaluated.
View Article and Find Full Text PDFSynergistic effect of mulch and nitrogen management on growth and essential oil yield of Salvia sclarea L.
Sci Rep
December 2024
Agrotechnology Division, Council of Scientific and Industrial Research - Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176 061, India.
Balanced plant nutrition and optimal micro-climate are critical for achieving higher production sustainably. Substituting mineral fertilizers with organic amendments under water-conserving strategies like mulch can enhance the quality and yield and improve soil health. Therefore, a two-year study was conducted to examine the synergistic effects of mulch and reducing inorganic fertilizers and partially substituting organic amendments on essential oil (EO) yield and its composition, and soil properties in Salvia sclarea, an industrially important crop.
View Article and Find Full Text PDFAtlas-scale single-cell DNA methylation profiling with sciMETv3.
Cell Genom
January 2025
Department of Molecular & Medical Genetics, Oregon Health & Science University, Portland, OR, USA; Cancer Early Detection Advanced Research Institute, Oregon Health & Science University, Portland, OR, USA; Knight Cardiovascular Institute, Oregon Health & Science University, Portland, OR, USA; Knight Cancer Institute, Oregon Health & Science University, Portland, OR, USA. Electronic address:
Single-cell methods to assess DNA methylation have not achieved the same level of cell throughput per experiment compared to other modalities, with large-scale datasets requiring extensive automation, time, and other resources. Here, we describe sciMETv3, a combinatorial indexing-based technique that enables atlas-scale libraries to be produced in a single experiment. To reduce the sequencing burden, we demonstrate the compatibility of sciMETv3 with capture techniques to enrich regulatory regions, as well as the ability to leverage enzymatic conversion, which can yield higher library diversity.
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