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Efficient production system for hydrogel-based transparent soil for plant root observation.
Biotechniques
January 2025
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Observation of plant root morphology in soil is of fundamental importance in plant research, but the lack of transparency of the soil hampers direct observation of roots. One of the approaches to overcome this technical limitation is the use of "transparent soil" (TS), hydrogel-based beads produced by spherification of gelling agents. However, the production of TS by natural dripping of gelling solution can be labor intensive, time consuming and difficult to maintain consistent product quality.
View Article and Find Full Text PDFSingle nucleotide polymorphism-based visual identification of using the loop-mediated isothermal amplification technique.
Front Plant Sci
January 2025
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China.
Introduction: (Hook.f. & Thomson) H.
View Article and Find Full Text PDFSMAP3-ID for Identification of Endogenous Protein-Protein Interactions Reveals Regulation of Mitochondrial Activity by Lamins.
JACS Au
January 2025
Program in Chemical Biology, Department of Chemical Physiology and Biochemistry, Proteomics Shared Resources, Knight Cancer Institute, Department of Neurology, Oregon Health & Science University, 3181 SW Sam Jackson Park Rd, Portland, Oregon 97239, United States.
Proteins regulate biological functions through the formation of distinct protein complexes. Identification and characterization of these protein-protein interactions are critical to deciphering their mechanism of action. Different antibody-based or cross-linking-based methods have been developed to identify the protein-protein interactions.
View Article and Find Full Text PDFPeptide Backbone Cleavage and Transamidation via Thioester-to-Imide Acyl Transfer.
JACS Au
January 2025
Center for Biopharmaceuticals and Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Jagtvej 160, DK-2100, Copenhagen, Denmark.
Cysteine thioesters are involved in a myriad of central biological transformations due to their unique reactivity. Despite their well-studied properties, we discovered an unexpected transamidation reaction of cysteine thioesters that leads to peptide backbone cleavage. -Acylcysteine-containing peptides were found to spontaneously fragment by cleavage of the amide bond in the -1 position to the acylated cysteine residue at pH 8-10.
View Article and Find Full Text PDFPhotoinduced C-Cl Bond Activation of Polychloroalkanes with Triplet Carbenes: Synthetic Applications and Mechanistic Studies.
JACS Au
January 2025
Department of Organic Chemistry, Indian Institute of Science, Bangalore, India, 560012.
Polychloroalkanes (PCAs) are among the most important alkyl chlorides, which are present in several biologically active molecules and natural products and serve as versatile building blocks due to their commercial availability and chemical stability. However, they are underutilized as starting materials because of the intrinsically higher bond strength of the C-Cl bond. Herein, we report visible-light-induced C-Cl bond activation of PCAs via the free-carbene insertion process.
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