An all optical technique based on near-infrared spectroscopy and mid-infrared imaging (MIRI) is applied as a noninvasive, in vivo tool to monitor the vascular status of skeletal muscle and the physiological changes that occur during exercise. A near-infrared spectroscopy (NIRS) technique, namely, steady state diffuse optical spectroscopy (SSDOS) along with MIRI is applied for monitoring the changes in the values of tissue oxygenation and thermometry of an exercising muscle. The NIRS measurements are performed at five discrete wavelengths in a spectral window of 650-850 nm and MIRI is performed in a spectral window of 8-12 microm. The understanding of tissue oxygenation status and the behavior of the physiological parameters derived from thermometry may provide a useful insight into muscle physiology, therapeutic response and treatment.
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