The study demonstrated the immunodiagnostic potential of differrent Egyptian human Schistosoma haematobium antigens. ELISA was used to measure the levels of total immunoglobulin G (IgG) and IgG4 antibodies (Abs) against S. haematobium adult worm antigens (Ags) (SAWA), excretory/secretory Ags (E/S) and cysteine proteinase Ag (27-29 kDa) for diagnosis of schistosomiasis. SDS-PAGE profiles of S. haematobium Ags showed several bands for SAWA, E/S and 27-29 kDa Ags which are characteristic of infections with Schistosoma spp. Purified protein fraction showed a single homogenous band of 27-29 kDa. For summarizing the potency of S. haematobium Ags, sensitivety rate, negative predictive value and diagnostic efficacy were calculated between data of 40 human patients in ELISA. SAWA Ag recorded 85.0 %, 77%, 90.0% with total IgG & 90.0 %, 83% and 93.3% with IgG4 isotype, respectively. While, E/S recorded 87.5%, 80%, 92.0% with total IgG and 92.5%, 87%, 95.0% with IgG4 isotype, respectively. Purified 27-29 kDa Ag presents the higher significant (P<0.01) results recording 90.0%, 83%, 93.3% with total IgG and 97.5%, 95%, 98.3% with IgG4 isotype, respectively. The results proved that combining the detection of IgG4 isotype using the 27-29 kDa Ag in sera of schistosomiasis haematobium patients in ELISA test could represent an effective immunodiagnostic tool for detecting infection in low worm burden population. This test could be useful in sero-epidemiolocal studies in low endemic areas and in diagnosis of infection in travelers to schistosomiasis endemic areas.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Properties of a fibrinolytic enzyme secreted by JS2 isolated from saeu (small shrimp) .
Food Sci Biotechnol
June 2018
1Division of Applied Life Science (BK21 Plus), Graduate School, Gyeongsang National University, Jinju, 52828 Korea.
species were screened to be used as starters for , salted and fermented Korean sea foods. A strain, JS2, showing strong fibrinolytic activity was isolated from saeu (small shrimp) , and identified as JS2 grew well at 20% (w/v) NaCl concentration. SDS-PAGE of culture supernatant from JS2 showed 3 major bands of 27, 29, and 60 kDa in size.
View Article and Find Full Text PDFLrHMGB1 Shares Structural Similarities with Human HMGB1, and Its Expression Is Induced in Bacterial Infection, Antiviral Vaccination, and Pathogen-Associated Molecular Patterns Stimulation.
DNA Cell Biol
August 2018
Immunology Laboratory, Fish Health Management Division, Indian Council of Agricultural Research-Central Institute of Freshwater Aquaculture, Bhubaneswar, Odisha, India .
The high-mobility group box 1 (HMGB1) protein is a highly conserved nonhistone chromosomal protein ubiquitously present in almost all cell types. In the nucleus, it facilitates DNA repair and replication, V(D)J recombination, stabilization of nucleosome, and in the cytoplasm, it regulates autophagy and apoptosis. In addition to these intracellular functions, HMGB1 also facilitates activation of innate immune responses and plays key roles in host defense.
View Article and Find Full Text PDFCharacterization of Cinnamoyl Esterases from Different Lactobacilli and Bifidobacteria.
Curr Microbiol
February 2017
Technische Universität München, Technische Mikrobiologie Weihenstephan, Gregor-Mendel-Straße 4, Freising, Germany.
A high variety of plants that are used for food production contain esterified hydroxycinnamic acids. As their free forms display several benefits, like an enhanced absorption in human intestinal tract, anti-oxidative and anti-carcinogenic effects, an improved protein solubility and reduced discoloration, the microbial ability to cleave the ester bond is highly desired. In order to examine potential fermentation strains for this purpose, six different lactic acid bacteria and one bifidobacterial strain were screened for their ability to degrade esterified hydroxycinnamic acids because these strains are commonly used for fermentation of plant-based foods.
View Article and Find Full Text PDFDetection of and common and uncommon antigens, using rabbit hyper immune serum raised against their excretory-secretory and somatic antigens.
J Parasit Dis
December 2016
Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Fasciolosis is an important neglected helminth disease caused by two liver flukes, and . The two species of are usually different in their morphological and molecular features. They have also common and uncommon antigens in both their somatic and excretory secretory metabolites.
View Article and Find Full Text PDFTuning Cationic Block Copolymer Micelle Size by pH and Ionic Strength.
Biomacromolecules
September 2016
Department of Chemistry, and ‡Department of Chemical Engineering & Materials Science, University of Minnesota, 207 Pleasant Street SE, Minneapolis, Minnesota 55455, United States.
The formation, morphology, and pH and ionic strength responses of cationic block copolymer micelles in aqueous solutions have been examined in detail to provide insight into the future development of cationic micelles for complexation with polyanions such as DNA. Diblock polymers composed of a hydrophilic/cationic block of N,N-dimethylaminoethyl methacrylate (DMAEMA) and a hydrophobic/nonionic block of n-butyl methacrylate (BMA) were synthesized [denoted as DMAEMA-b-BMA (X-Y), where X = DMAEMA molecular weight and Y = molecular weight of BMA in kDa]. Four variants were created with block molecular weights of 14-13, 14-23, 27-14, 27-29 kDa and low dispersities less than 1.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!