We report an experimental and theoretical study of the radial elasticity of tobacco mosaic virus (TMV) nanotubes. An atomic force microscope tip is used to apply small radial indentations to deform TMV nanotubes. The initial elastic response of TMV nanotubes can be described by finite-element analysis in 5nm indentation depths and Hertz theory in 1.5nm indentation depths. The derived radial Young's modulus of TMV nanotubes is 0.92+/-0.15GPa from finite-element analysis and 1.0+/-0.2GPa from the Hertz model, which are comparable with the reported axial Young's modulus of 1.1GPa [Falvo, Biophys. J. 72, 1396 (1997)].
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http://dx.doi.org/10.1103/PhysRevE.78.031914 | DOI Listing |
Macromol Biosci
March 2024
Institute of Interfacial Process Engineering and Plasma Technology IGVP, University of Stuttgart, Nobelstr. 12, 70569, Stuttgart, Germany.
An all-soft multi-material combination consisting of a hydrogel based on poly(ethylene glycol) (PEG) coated with spatially defined spots of gelatin methacryloyl (GM) containing selectively addressable viral nanorods is presented, and its basic application as a qualitative biosensor with reporter enzymes displayed on the tobacco mosaic virus (TMV) bioscaffolds within the GM is demonstrated. Biologically inert PEG supports are equipped with GM spots serving as biological matrix for enzymes clustered on TMV particles preventing diffusion out of the gel. For this multi-material combination, i) the PEG-based hydrogel surface is modified to achieve a clear boundary between coated and non-coated regions by introducing either isothiouronium or thiol groups.
View Article and Find Full Text PDFJ Mol Biol
December 2022
Biochemistry and Metabolism, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK. Electronic address:
We have investigated whether the presence of the origin of assembly sequence (OAS) of tobacco mosaic virus (TMV) is necessary for the specific encapsidation of replicating viral RNA. To this end TMV coat protein was expressed from replicating RNA constructs with or without the OAS in planta. In both cases the replicating RNA was specifically encapsidated to give nucleoprotein nanorods, though the yield in the absence of the OAS was reduced to about 60% of that in its presence.
View Article and Find Full Text PDFAtherosclerosis is a progressive cardiovascular disease in which cholesterol-rich plaques build up within arteries, increasing the risk of thrombosis, myocardial infarction, and stroke. One promising therapeutic approach is the use of high-density lipoprotein (HDL) biomimetic formulations based on ApoAI peptides that promote cholesterol efflux from plaques, ultimately leading to cholesterol excretion. Here, we describe the multivalent display of ApoAI peptides on the surface of protein nanotubes derived from the plant virus tobacco mosaic virus (TMV) and protein nanoparticles using virus-like particles from bacteriophage Qβ.
View Article and Find Full Text PDFBiomacromolecules
October 2022
Department of NanoEngineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093, United States.
Ovarian cancer is the foremost cause of gynecological cancer and a major cause of cancer death in women. Treatment for advanced stage is surgical debulking followed by chemotherapy; however, most patients relapse with more aggressive and therapy-resistant tumors. There is a need to develop drug delivery approaches to deliver platinum therapies to tumors to increase efficacy while maintaining safety.
View Article and Find Full Text PDFMol Biotechnol
June 2022
Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory, Faculty of Science, and Stem Cell Institute, Ankara University, Ankara, Turkey.
To date, metallization studies have been performed with the nanometer-scale template, Tobacco Mosaic Virus (TMV). Here we show that fullerenes as well can be deposited on TMV coat protein in a controlled manner. Two methods were followed for the coating process.
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