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Directed Evolution of Fluorescent Genetically Encoded Biosensors: Innovative Approaches for Development and Optimization of Biosensors.
Chembiochem
March 2025
Sirius university of Science and Technology, Research Center for Translational Medicine, Olympic Ave., 1, 354340, Sochi, RUSSIAN FEDERATION.
Fluorescent protein-based biosensors are indispensable molecular tools in cell biology and biomedical research, providing non-invasive dynamic measurements of metabolite concentrations and other cellular signals. Traditional methods for developing these biosensors rely on rational design, but directed evolution methods offer a more efficient alternative. This review discusses recent advancements in the development of biosensors using directed evolution, including methods for optimizing domain fusions, sequence optimization, and new screening and selection systems.
View Article and Find Full Text PDFDynamics of ammonia-oxidizing microorganisms in gradient temperature-regulated reactors under low ammonia loading condition.
J Environ Manage
March 2025
Department of Environmental and Sustainable Engineering, Faculty of Engineering, Chulalongkorn University, Bangkok, Thailand; Biotechnology for Wastewater Engineering Research Unit, Chulalongkorn University, Bangkok, Thailand. Electronic address:
Ammonia-oxidizing microorganisms (AOMs) play a crucial role in nitrogen removal in engineered systems. However, temperature fluctuations can lead to ammonia oxidation failure. This study investigated the effects of a broad temperature range (10.
View Article and Find Full Text PDFAuxin-responsive OsMADS60 negatively mediates rice tillering and grain yield by modulating OsPIN5b expression.
Plant J
March 2025
Institute of Rice Industry Technology Research, Key Laboratory of Functional Agriculture of Guizhou Provincial Higher Education Institutions, Key Laboratory of Molecular Breeding for Grain and Oil Crops in Guizhou Province, College of Agricultural Sciences, Guizhou University, Guiyang, Guizhou, 550025, China.
Rice tillering determines grain yield, yet the molecular regulatory network is still limited. In this study, we demonstrated that the transcription factor OsMADS60 promotes the expression of the auxin transporter OsPIN5b to affect auxin distribution and inhibit rice tillering and grain yield. Natural variation was observed in the promoter region of OsMADS60, with its expression level negatively correlated with tiller number and inducible by auxin.
View Article and Find Full Text PDFPhylogenetic analysis and transmission networks highlight the role of older adults in the transmission of HIV-1 in northern Zhejiang, China.
Virol J
March 2025
Department of AIDS and STD Prevention and Control, Zhejiang Provincial Center for Disease Control and Prevention, 3399 Binsheng Road, Binjiang District, Hangzhou, Zhejiang Province, 310051, P. R. China.
Background: In China, human immunodeficiency virus (HIV) poses a significant challenge to older heterosexual adults. This study explored the transmission dynamics of HIV infection among older adults in northern Zhejiang Province, China using phylogenetic analysis and transmission networks.
Methods: HIV pol sequences without any antiretroviral therapy were obtained from newly diagnosed HIV-positive patients in Huzhou between 2017 and 2022.
Next-generation sequencing-based population genetics unravels the evolutionary history of Rhodomyrtus tomentosa in China.
BMC Plant Biol
March 2025
College of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, 510220, China.
Background: Rhodomyrtus tomentosa (Ait.) Hassk. is useful for its ornamental, medicinal, and ecological characteristics, and is considered a "Neglected and Underutilized Crop Species".
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