Hepatocyte nuclear factor 4 alpha (HNF4 alpha) is a member of the nuclear receptor superfamily and is expressed in several endodermal tissues. The aim of the present study was to examine the expression of HNF4 alpha on ovarian epithelial tumors with immunocytochemistry and immunohistochemistry using mAbs recognizing P1 and P2 promoter-driven HNF4 alpha. Ovarian mucinous adenoma, mucinous tumors of borderline malignancy, and mucinous adenocarcinoma had positive nuclear staining for HNF4 alpha (41/45, 91%). One-third (34%) of mucinous tumors had P1-positive staining and most had P1/P2-positive staining (93%). MUC2- and MUC5AC-positive staining was observed in 34% and 95% of mucinous tumors, respectively. The histological subtype of these mucinous tumors was not correlated with HNF4 alpha expression. On cytology it was found that cancer cells in the ascites from ovarian mucinous adenocarcinomas were HNF4 alpha positive, but tumor cells in ascites from other types of ovarian carcinomas were negative for HNF4 alpha. Thus, HNF4 alpha is demonstrated to be a useful marker for histological and cytological diagnosis of ovarian mucinous tumors.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/j.1440-1827.2008.02293.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Dengue virus NS1 leads to downregulation of HNF4 alpha in liver cells resulting in a decrease in coagulation factors I, V, X, and XIII, contributing to coagulopathy.
J Virol
December 2024
CSIR-Indian Institute of Chemical Biology, Kolkata, West Bengal, India.
Unlabelled: Dengue virus NS1 protein is a major pathogenic protein. In this study, we examined the role of NS1 in coagulopathy associated with Dengue infection, a common feature of Dengue virus pathogenesis. Since most coagulation factors are produced by hepatocytes and liver is key organ affected during infection, we conducted transcriptomics using total-RNA extracted from Huh7 cells overexpressing NS1 protein.
View Article and Find Full Text PDFLoss of ARID3A perturbs intestinal epithelial proliferation-differentiation ratio and regeneration.
J Exp Med
October 2024
Stem Cell and Cancer Biology Laboratory, The Francis Crick Institute, London, UK.
Intestinal stem cells at the crypt divide and give rise to progenitor cells that proliferate and differentiate into various mature cell types in the transit-amplifying (TA) zone. Here, we showed that the transcription factor ARID3A regulates intestinal epithelial cell proliferation and differentiation at the TA progenitors. ARID3A forms an expression gradient from the villus tip to the upper crypt mediated by TGF-β and WNT.
View Article and Find Full Text PDFAcanthopanax senticosus ameliorates steatohepatitis through HNF4 alpha pathway activation in mice.
Sci Rep
January 2024
Department of Gastroenterology and Oncology, Tokushima University Graduate School of Biomedical Sciences, Tokushima, 770-0042, Japan.
Article Synopsis
- * A study using mice on a high-fat diet showed that those given Acanthopanax senticosus Harms extract (ASH) had less liver fat, inflammation, and fibrosis after 6 weeks.
- * The extract activated a key liver transcription factor, HNF4 alpha, leading to increased fat secretion from liver cells, suggesting ASH may be a preventative treatment for fatty liver disease.
Nuclear receptors including Aryl hydrocarbon Receptor (AhR), Constitutive Androstane Receptor (CAR), Pregnane X Receptor (PXR), and Peroxisome Proliferator-Activated Receptor-alpha (PPARα) function as xenobiotic sensors. Hepatocyte nuclear factor 4alpha (HNF4α) is a highly conserved orphan nuclear receptor essential for liver function. We tested the hypothesis that HNF4α is essential for function of these four major xenosensors.
View Article and Find Full Text PDFNuclear hormone receptor NHR-49 is an essential regulator of stress resilience and healthy aging in .
Front Physiol
August 2023
Centre for Molecular Medicine and Therapeutics, The University of British Columbia, Vancouver, BC, Canada.
The genome of encodes 284 nuclear hormone receptor, which perform diverse functions in development and physiology. One of the best characterized of these is NHR-49, related in sequence and function to mammalian hepatocyte nuclear factor 4α and peroxisome proliferator-activated receptor . Initially identified as regulator of lipid metabolism, including fatty acid catabolism and desaturation, additional important roles for NHR-49 have since emerged.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!