Results of experimental researches in the modelling systems simulating infringements of temperature modes of storage of a grain, shown an opportunity of intensive accumulation mycotoxin citrinin in infected by toxigenic molds Penicillium citrinum grains of rice and wheat are submitted. Most high levels of citrinin were found out as a result of growth P. citrinum in rice, achieving 300-350 mg/kg of a grain during 21 days. High frequency of occurrence among Penicillium genera citrinin-producing strains (5.9%) was established, that specifies a large extent of risk for human health of food grains, contaminated this widespread kind of moldsopportunity of application ELISA method with using "RIDASCREEN Citrinin" kits for detection and quantification of citrinin in a food grain is shown.

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